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Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 35512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV9 | 35512-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Backbone sizew/o insert(bp)5385
- Modifications to backboneAddition of a CaMKIIa promoter and a WPRE
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert namehChR2
- SpeciesChlamydomonas reinhardtii
- Insert Size (bp)1646
- MutationE123T and T159C
- PromoterCaMKIIa
- Tag/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteBamHI(not destroyed)
- 3′ cloning siteEcoRI(not destroyed)
- 5′ sequencing primerAGTCCTGCAGTATTGTGTAT
- 3′ sequencing primerGCAATAGCATGATACAAAGG (Common Sequencing Primers)
Resource Information
- Terms and Licenses
- UBMTA
- Takara Bio Limited Use Label License (formerly Clontech)
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 3 References
Depositor Comments
Information for AAV9 (Catalog # 35512-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry (#35512). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry plasmid DNA.
CaMKIIa-driven, humanized channelrhodopsin E123T/T159C mutant fused to mCherry for optogenetic activation.These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
- Reporter GenemCherry
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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2.溶氧,在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一,有便于对不同参数的取样测定。。
水浴恒温摇床可分为常温水浴恒温摇床(室温~100℃)和冷冻水浴恒温摇床(常用为0~100℃,也可定制更低温度的如:-10℃~100℃)。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为:室温+5~60℃。冷冻气浴恒温摇床的温度范围为:4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
ZD-85双功能气浴恒温摇床型号:
(1)CHA-S型、恒温气浴摇床:属于-往复式
(2)SHZ-82型、恒温气浴摇床:属于-回旋式
(3)ZD-85型、恒温气浴摇床:属于-往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数:
1、 使用电源: 220V 50Hz
2、 加热功率: 400w
3、 定时范围: 0~120分(或常开)
4、 振荡频率: 起动—300转/分,可调
5、 振荡幅度: 20mm
6、 恒温范围: 室温—50℃
7、 振荡方法: 往复、回旋和双功能(采购时选择)
8: 温控精度: +0.5℃
9: 装瓶量:试管:16×300mm 100ml×24只、200ml×15只
10: 外形尺寸: 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性:≤±0.5℃
温控范围:室温-100 ℃
温度波动:±0.5℃
加热功率:1800W
振荡频率:起动-280次/分
振幅:20mm
定时范围:0-120分(或常开)
工作电源:AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5~60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃~40℃
输入功率750W
内胆尺寸(W*D*H)mm1120*640*830mm(595L)
外形尺寸(W*D*H)mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0~999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开

