Recombinant Human His6-HR23A/Rad23A Tandem UBA (TUBE1), CF Summary
Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within thealpha 1 andalpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs,have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).
This protein is His6-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies.
Specifications
- 12 months from date of receipt, -70 °C as supplied.
- 3 months, -70 °C under sterile conditions after opening.
Product Datasheets
Background: HR23A/Rad23A TUBE1
Reagents Provided
FAQs
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1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
2,适宜的床面横向坡度。
3,冲洗水大小要适宜。
4,给矿量适当切均匀。
5,给矿浓度适宜。
6,物料在入选前的准备。
7,物料在床面的分带和产品的截取。
操作的时候还有一些细节,有什么问题可以咨询河南红星机器,摇床生产出口基地,30年的生产经验,可以免费安装调试维修。
培养皿上的细菌是一个月前接种的,培养基成分没有变化
如题,WB中,脱脂牛奶封闭时,忘记使用脱色摇床摇晃,对封闭效果影响大不大?有没有什么补救办法?延长封闭时间还是怎么办?急等,谢谢!!!!

