DescriptionViPrimePLUS AtTaq qPCR Green Master Mix II is next generation first choice mix designed for fast and easy real-time PCR reaction set up. The improved formulation of master mix contains pure Hot Start Taq DNA Polymerases, EvaGreen® dye, highest quality dNTPs, and buffer components at optimal concentrations. Hot Start Taq DNA Polymerases in the master mix provide antibody mediated hot start mechanism which releases more active enzyme and requires shorter activation time. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS AtTaq qPCR Green Master Mix II can be used to amplify any DNA template including genomic, cDNA and viral sequences. The improved formulation of qPCR green master mix can detect extremely low copy number targets very specifically with high efficiency. The qPCR green master mix is designed to prevent and reduce the formation of primer dimers and non-specific products leading to optimum sensitivity and specificity.ViPrimePLUS AtTaq qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS AtTaq qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of sample material suited for qPCR amplification can be used.
Features
- Fast and easy real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Contain Hot Start Taq DNA Polymerase - highest sensitivity and specificity
- Contain EvaGreen® dye – highest dye stability and safety
- Increased limit of detection
- Minimal PCR inhibition
- Compatible with most of the real-time PCR platforms
Component1.6ml aliquots of master mix
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS AtTaq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM07ViPrimePLUS AtTaq qPCR Green Master Mix II (EvaGreen® Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM07-LRViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX (EvaGreen® Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM07-RViPrimePLUS AtTaq qPCR Green Master Mix II with ROX (EvaGreen® Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM07 | ViPrimePLUS AtTaq qPCR Green MasterMix II (EvaGreen® Dye) | 150 reactions |
| QLMM07-LR | ViPrimePLUS AtTaq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) | 150 reactions |
| QLMM07-R | ViPrimePLUS AtTaq qPCR Green MasterMix II with ROX (EvaGreen® Dye) | 150 reactions |
DownloadManual
ViPrimePLUS AtTaq qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS AtTaq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS AtTaq qPCR Green MasterMix II with ROX (EvaGreen® Dye)
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ZD-85双功能气浴恒温摇床型号：
（1）CHA－S型、恒温气浴摇床：属于－往复式
（2）SHZ-82型、恒温气浴摇床：属于－回旋式
（3）ZD－85型、恒温气浴摇床：属于－往复和回旋式、双功能。
ZD-85双功能气浴恒温摇床主要技术参数：
1、 使用电源： 220V 50Hz
2、 加热功率： 400w
3、 定时范围： 0~120分（或常开）
4、 振荡频率： 起动—300转/分,可调
5、 振荡幅度： 20mm
6、 恒温范围： 室温—50℃
7、 振荡方法： 往复、回旋和双功能（采购时选择）
8： 温控精度： +0.5℃
9： 装瓶量：试管：16×300mm 100ml×24只、200ml×15只
10： 外形尺寸： 700×470×500mm
THZ-82水浴恒温摇床技术指标
温控均匀性：≤±0.5℃
温控范围：室温-100 ℃
温度波动：±0.5℃
加热功率：1800W
振荡频率：起动-280次/分
振幅：20mm
定时范围：0-120分(或常开)
工作电源：AC220V 50Hz
水浴恒温摇床型号与振荡方式
SHA-C型为往复水浴恒温摇床
THZ-82型为回旋水浴恒温摇床
SHA-B型为双功能水浴恒温摇床
大容量恒温摇床参数 产品名称
双层恒温摇床
产品型号TS-1112B
显示方式液晶一屏显示
电源电压AC220V 50HZ
控温范围室温+5～60℃
温度分辨率0.1℃
照明方式内置照明灯
制冷方式自动
压缩机化霜方式自动
波动度±0.1℃
旋转频率30-280rpm
转速精度±1rpm
摆振幅度0-50mm无极可调
标准配置250ml*45支 500ml*36支
最大容量250ml*90支或50ml*72支或1000ml*36支
托盘尺寸970*560mm
震荡方式回旋式
工作环境温度5℃～40℃
输入功率750W
内胆尺寸（W*D*H）mm1120*640*830mm（595L）
外形尺寸（W*D*H）mm1200*800*150mm
拖盘数量2块
净重360KG
定时范围0～999小时
参考资料:Hi.baidu/=。www.jingda17.net。DI展开
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
培养皿上的细菌是一个月前接种的，培养基成分没有变化
水浴恒温摇床可分为常温水浴恒温摇床（室温~100℃）和冷冻水浴恒温摇床（常用为0~100℃，也可定制更低温度的如：-10℃~100℃）。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为：室温+5~60℃。冷冻气浴恒温摇床的温度范围为：4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
2.溶氧，在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一，有便于对不同参数的取样测定。。
如题，WB中，脱脂牛奶封闭时，忘记使用脱色摇床摇晃，对封闭效果影响大不大？有没有什么补救办法？延长封闭时间还是怎么办？急等，谢谢！！！！
