DescriptionViPrimePLUS AtTaq qPCR Green Master Mix II is next generation first choice mix designed for fast and easy real-time PCR reaction set up. The improved formulation of master mix contains pure Hot Start Taq DNA Polymerases, EvaGreen® dye, highest quality dNTPs, and buffer components at optimal concentrations. Hot Start Taq DNA Polymerases in the master mix provide antibody mediated hot start mechanism which releases more active enzyme and requires shorter activation time. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS AtTaq qPCR Green Master Mix II can be used to amplify any DNA template including genomic, cDNA and viral sequences. The improved formulation of qPCR green master mix can detect extremely low copy number targets very specifically with high efficiency. The qPCR green master mix is designed to prevent and reduce the formation of primer dimers and non-specific products leading to optimum sensitivity and specificity.ViPrimePLUS AtTaq qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS AtTaq qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of sample material suited for qPCR amplification can be used.
Features
- Fast and easy real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Contain Hot Start Taq DNA Polymerase - highest sensitivity and specificity
- Contain EvaGreen® dye – highest dye stability and safety
- Increased limit of detection
- Minimal PCR inhibition
- Compatible with most of the real-time PCR platforms
Component1.6ml aliquots of master mix
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS AtTaq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM07ViPrimePLUS AtTaq qPCR Green Master Mix II (EvaGreen® Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM07-LRViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX (EvaGreen® Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM07-RViPrimePLUS AtTaq qPCR Green Master Mix II with ROX (EvaGreen® Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM07 | ViPrimePLUS AtTaq qPCR Green MasterMix II (EvaGreen® Dye) | 150 reactions |
| QLMM07-LR | ViPrimePLUS AtTaq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) | 150 reactions |
| QLMM07-R | ViPrimePLUS AtTaq qPCR Green MasterMix II with ROX (EvaGreen® Dye) | 150 reactions |
DownloadManual
ViPrimePLUS AtTaq qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS AtTaq qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS AtTaq qPCR Green MasterMix II with ROX (EvaGreen® Dye)
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如题，WB中，脱脂牛奶封闭时，忘记使用脱色摇床摇晃，对封闭效果影响大不大？有没有什么补救办法？延长封闭时间还是怎么办？急等，谢谢！！！！
2，适宜的床面横向坡度。
3，冲洗水大小要适宜。
4，给矿量适当切均匀。
5，给矿浓度适宜。
6，物料在入选前的准备。
7，物料在床面的分带和产品的截取。
操作的时候还有一些细节，有什么问题可以咨询河南红星机器，摇床生产出口基地，30年的生产经验，可以免费安装调试维修。
1.传质。就是底物或代谢产物更好在体系内转移和发挥作用。
2.溶氧。在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。
3.体系均一。有便于对不同参数的取样测定。
水浴恒温摇床可分为常温水浴恒温摇床（室温~100℃）和冷冻水浴恒温摇床（常用为0~100℃，也可定制更低温度的如：-10℃~100℃）。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为：室温+5~60℃。冷冻气浴恒温摇床的温度范围为：4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
2.溶氧，在好氧培养过程中，空气是滤过开放的，所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一，有便于对不同参数的取样测定。。
