Coronavirus COVID-19 IgG ELISA Assay
Coronavirus COVID-19 IgG ELISA Assay was developed and manufactured in the US
Size: 1×96 wells
Sensitivity: Cut-off control
Incubation Time: 1.5 Hours
Sample Type: Serum
Species Sample: Human
Sample Size: 10 µL
Alternative Names: Serology, Coronavirus, COVID-19, Serological
IVD, CE-Marked
Controls Included
Cross Reactivity
Panels were studied with a minimum of five confirmed disease state samples with this serology ELISA assay. No interference was observed for the following disease or infectious agents:
- Anti-influenza A
- Anti-influenza B
- Hepatitis C (HCV)
- Antinuclear Antibodies (ANA)
- Respiratory Syncytial (RSV)
Assay Principle
The COVID-19 IgG ELISA Assay is designed, developed, and produced for the qualitative measurement of the COVID-19 IgG antibody in serum samples (serology). This serological assay utilizes the microplate based enzyme immunoassay technique.
Assay controls and samples are added to the microtiter wells of a microplate that was coated with the COVID-19 peptide antigen nucleocapsid protein. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase labeled COVID-19 IgG tracer antibody is added to each well. After an incubation period, an immunocomplex of ” COVID-19 polypeptide antigen – new coronavirus IgG antibody HRP labeled COVID-19 IgG tracer antibody ” is formed if there is coronavirus IgG antibody present in the tested materials. The unbound tracer antibody is removed by the subsequent washing step. HRP labeled tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the coronavirus IgG on the wall of the microtiter well is proportional to the amount of the coronavirus IgG antibody level in the tested materials.
Information for US Customers
During this crisis, steps are being taken to ensure that we can provide access to quality products for diagnostic testing. The Food and Drug Administration (FDA) has issued guidance which allows for distribution of the serological tests. At this time, the kits are released for sale in the United States under the provisions outlined in Section D of Policy for Diagnostic Tests for Coronavirus Disease-2019 during the Public Health Emergency.
The kits are registered under product code QKO, the submission number is D376537.
Per the aforementioned guidance, the following statements are required:
- This test has not been reviewed by the FDA.
- Negative results do not rule out SARS-CoV-2 infection, particularly in those who have been in contact with the virus.
- Follow-up testing with a molecular diagnostic should be considered to rule out infection in these individuals.
- Results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status.
- Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.
This kit is only provided for use by clinical laboratories or to healthcare workers for point-of-care testing, and not for at home testing.
Products Related to Coronavirus COVID-19 IgG ELISA Assay
Coronavirus COVID-19 IgM ELISA
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二、限制性内切酶消化样品DNA
三、凝胶电泳分离消化产物
四、如果靶序列>5kb，在0.25M的HCl中进行震荡脱嘌呤10min，ddH2O洗一次
五、用变性液震荡处理30min，ddH2O洗一次
六、中和液震荡处理30min
七、裁取合适大小的尼龙膜或硝酸纤维素膜进行转膜操作，可用真空转膜仪或者搭滤纸桥，需要20×SSC
八、制备探针，可用地高辛标记（以地高辛为例）
九、将膜放入杂交瓶，42°C预杂交30min
十、倒掉预杂交液，加入杂交液，适当的温度进行杂交4h至过夜
十一、洗膜，先用2×SSC+0.1%SDS，20-25°C洗2×5min，再用0.5×SSC+0.1%SDS洗2×15min。然后用washing buffer洗1-5min，接着用blocking solution洗30min后，用antibody solution洗30min，再用washing buffer洗2×15min，再用detection buffer洗2-5min后，取出膜，放于保鲜膜上，在结合有DNA的一面滴加CSPD ready-to-use后，立刻盖上保鲜膜，让CSPD ready-to-use均匀的布满膜表面，室温放置5min后，37°C温育10min以上
十二、放射自显影，可用成像系统信号累积模式显影或用X-ray显影
应该注意以下2个方面
1 用玻璃棒将气泡赶净 有气泡的位置不导电 蛋白就转不上
2 半干式转膜仪要保证一定量的转移缓冲液，如果转膜一个小时以上，缓冲液要多一些
我用Bio-rda转膜仪，转膜2分钟就停止，然后机器上显示“noloaddetected“。各位知道这是为什么吗？？
第一次出现这种情况，同样的条件，之前一直没有问题。
