- Choose an extended warranty option for yourAutomated TEER Measurement System (SYS-REMS)
- One year or two years coverage
- Comprehensive, no-questions-asked protection from accidental damage to your equipment
- Minimize downtime with 5-day turnaround time on service
- Free annual cleaning, calibration and repair, along with a Calibration/Verification Certificate
- Avoid the expenses related to accidentally damaged equipment
- Free ground shipping to and from the WPI Service Center
- See the extended warranty details
Activate Your New Product Warranty
Options
| Order code | Description |
| SYS-REMS-EX1 | SYS-REMS Extended Warranty 1-Year |
| SYS-REMS-EX2 | SYS-REMS Extended Warranty 2-Year |
WPI"s extended warranties provide peace of mind when unexpected events occur. This plan"s coverage exceeds the standard manufacturer"s warranty and includes accidental damage to your equipment. You choose:
- 2-Year Comprehensive, No Question Asked Warranty Coverage
- 1-Year Comprehensive, No Question Asked Warranty Coverage
NOTE: The Extended Warranty can be bought only at the time of purchase. Purchase of the warranty includes electronic notification of free annual calibration/verification.
Activate Your New Product Warranty
Terms & Conditions of the Extended Warranty
Warranty Brochure
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二、限制性内切酶消化样品DNA
三、凝胶电泳分离消化产物
四、如果靶序列>5kb,在0.25M的HCl中进行震荡脱嘌呤10min,ddH2O洗一次
五、用变性液震荡处理30min,ddH2O洗一次
六、中和液震荡处理30min
七、裁取合适大小的尼龙膜或硝酸纤维素膜进行转膜操作,可用真空转膜仪或者搭滤纸桥,需要20×SSC
八、制备探针,可用地高辛标记(以地高辛为例)
九、将膜放入杂交瓶,42°C预杂交30min
十、倒掉预杂交液,加入杂交液,适当的温度进行杂交4h至过夜
十一、洗膜,先用2×SSC+0.1%SDS,20-25°C洗2×5min,再用0.5×SSC+0.1%SDS洗2×15min。然后用washing buffer洗1-5min,接着用blocking solution洗30min后,用antibody solution洗30min,再用washing buffer洗2×15min,再用detection buffer洗2-5min后,取出膜,放于保鲜膜上,在结合有DNA的一面滴加CSPD ready-to-use后,立刻盖上保鲜膜,让CSPD ready-to-use均匀的布满膜表面,室温放置5min后,37°C温育10min以上
十二、放射自显影,可用成像系统信号累积模式显影或用X-ray显影
应该注意以下2个方面
1 用玻璃棒将气泡赶净 有气泡的位置不导电 蛋白就转不上
2 半干式转膜仪要保证一定量的转移缓冲液,如果转膜一个小时以上,缓冲液要多一些
我用Bio-rda转膜仪,转膜2分钟就停止,然后机器上显示“noloaddetected“。各位知道这是为什么吗??
第一次出现这种情况,同样的条件,之前一直没有问题。

