
Anti-Mouse IgG Antibody
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Product Description | The antibody is developed from goats immunized with mouse immunoglobulin G (IgG), and affinity purified using mouse IgG conjugated agarose. The purified antibody can be used for detection, identification, quantization, localization and affinity purification of mouse IgG from mouse serum, ascites and mouse hybridoma cell culture medium, etc. | Sample Data Immunohistochemical staining using anti-Mouse IgG Antibody (ICP9401) as the secondary antibody | |
Species | Goat | ||
Formulation | 2 mg/mL in PBS or lyophilized powder | ||
Immunogen | Purified mouse IgG | ||
Purification | The antibody is affinity purified with mouse IgG conjugated agarose and absorbed with human IgG conjugated agarose. | ||
Specificity | The antibody is specific to mouse IgG, may slightly cross-react to rat IgG | ||
Applications | ELISA WB IHC IAC | ||
Scientific Description | IgG is a monomeric immunoglobulinthatconsists of two heavy chains and two light chains. Each IgG molecule has two antigen binding sites. IgG is the most abundant immunoglobulin in the blood and tissue. It provides protection against many kinds of bacteria, viruses and fungi, and plays an important role in cell phagocytosis and bacterial toxin neutralization. | ||
Storage & Stability | Product is stable for 30 daysatroom temperature. For extended storage, store product at –20ºC. Expiration date is three yearsfromthedate of shipping if properly stored. |
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二、限制性内切酶消化样品DNA
三、凝胶电泳分离消化产物
四、如果靶序列>5kb,在0.25M的HCl中进行震荡脱嘌呤10min,ddH2O洗一次
五、用变性液震荡处理30min,ddH2O洗一次
六、中和液震荡处理30min
七、裁取合适大小的尼龙膜或硝酸纤维素膜进行转膜操作,可用真空转膜仪或者搭滤纸桥,需要20×SSC
八、制备探针,可用地高辛标记(以地高辛为例)
九、将膜放入杂交瓶,42°C预杂交30min
十、倒掉预杂交液,加入杂交液,适当的温度进行杂交4h至过夜
十一、洗膜,先用2×SSC+0.1%SDS,20-25°C洗2×5min,再用0.5×SSC+0.1%SDS洗2×15min。然后用washing buffer洗1-5min,接着用blocking solution洗30min后,用antibody solution洗30min,再用washing buffer洗2×15min,再用detection buffer洗2-5min后,取出膜,放于保鲜膜上,在结合有DNA的一面滴加CSPD ready-to-use后,立刻盖上保鲜膜,让CSPD ready-to-use均匀的布满膜表面,室温放置5min后,37°C温育10min以上
十二、放射自显影,可用成像系统信号累积模式显影或用X-ray显影