Anti-Mouse IgG Antibody
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Product Description | The antibody is developed from goats immunized with mouse immunoglobulin G (IgG), and affinity purified using mouse IgG conjugated agarose. The purified antibody can be used for detection, identification, quantization, localization and affinity purification of mouse IgG from mouse serum, ascites and mouse hybridoma cell culture medium, etc. | Sample Data Immunohistochemical staining using anti-Mouse IgG Antibody (ICP9401) as the secondary antibody | |
Species | Goat | ||
Formulation | 2 mg/mL in PBS or lyophilized powder | ||
Immunogen | Purified mouse IgG | ||
Purification | The antibody is affinity purified with mouse IgG conjugated agarose and absorbed with human IgG conjugated agarose. | ||
Specificity | The antibody is specific to mouse IgG, may slightly cross-react to rat IgG | ||
Applications | ELISA WB IHC IAC | ||
Scientific Description | IgG is a monomeric immunoglobulinthatconsists of two heavy chains and two light chains. Each IgG molecule has two antigen binding sites. IgG is the most abundant immunoglobulin in the blood and tissue. It provides protection against many kinds of bacteria, viruses and fungi, and plays an important role in cell phagocytosis and bacterial toxin neutralization. | ||
Storage & Stability | Product is stable for 30 daysatroom temperature. For extended storage, store product at –20ºC. Expiration date is three yearsfromthedate of shipping if properly stored. |
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应该注意以下2个方面
1 用玻璃棒将气泡赶净 有气泡的位置不导电 蛋白就转不上
2 半干式转膜仪要保证一定量的转移缓冲液,如果转膜一个小时以上,缓冲液要多一些
搞核酸的:PCR仪器,电泳槽,电泳仪,凝胶扫描设备
搞蛋白的:各类电泳设备,转膜仪,摇床,有需要还要特别搞一个暗房间
基本就这些吧,话说,兄台真要筹建一个实验室,为何不亲自到一个实验室转一圈呢?