
Product information | Protocols | References | Technical support | Reviews |
Product overview
Product name | Xanthurenic acid polyclonal antibody |
Synonyms | Anti-Xanthurenic acid polyclonal antibody 8-Hydroxy-4-oxo-1H-quinoline-2-carboxylic acid polyclonal antibody Xanthuric acid polyclonal antibody Xanthurenate polyclonal antibody 8-Hydroxykynurenic acid polyclonal antibody |
Immunogen | Conjugated Xanthurenic acid |
Specificity | When tested in competitive ELISA, the anti-conjugated Xanthurenic acid antibody did not display any significant cross-reactivity with analog Kynurenic acid conjugate. |
Lot number | 140801 |
Reconstitution & storage
Form | Liquid |
Purity | Purified anti-serum |
Storage | Store at 4°C |
Storage buffer | Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
Material safety datasheet | Download MSDS |
Protocols
Immunohistochemistry (IHC) | Optimal working conditions must be determined by the end-user. |
Immunofluorescence (IF) | Optimal working conditions must be determined by the end-user. |
Restrictions | For research use only |
References
- Product citation : Roussel et al. Xanthurenic acid is localized in neurons in the central nervous system. Neuroscience. 2016 Aug 4
Selected publications on Xanthurenic acid:
- Neale SA, Copeland CS, Salt TE. Effect of VGLUT inhibitors on glutamatergic synaptic transmission in the rodent hippocampus and prefrontal cortex.Neurochem Int. 2013 Oct 10. pii: S0197-0186(13)00250-7. doi: 10.1016/j.neuint.2013.10.001. [Epub ahead of print]
- NealeSA et al. Modulation ofhippocampalsynaptic transmission by the kynurenine pathway memberxanthurenic acidand other VGLUT inhibitors. Neuropsychopharmacology.2013May;38(6):1060-7. doi: 10.1038/npp.2013.4. Epub 2013 Jan 7.
- Copeland CS1, Neale SA, Salt TE. Actions of Xanthurenic acid, a putative endogenous Group II metabotropic glutamate receptor agonist, on sensory transmission in the thalamus.Neuropharmacology. 2013 Mar;66:133-42. doi: 10.1016/j.neuropharm.2012.03.009. Epub 2012 Apr 2.
- Taleb O et al. Xanthurenic acid binds to neuronal G-protein-coupled receptors that secondarily activate cationic channels in the cell line NCB-20.PLoS One. 2012;7(11):e48553. doi: 10.1371/journal.pone.0048553. Epub 2012 Nov 6.
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二、限制性内切酶消化样品DNA
三、凝胶电泳分离消化产物
四、如果靶序列>5kb,在0.25M的HCl中进行震荡脱嘌呤10min,ddH2O洗一次
五、用变性液震荡处理30min,ddH2O洗一次
六、中和液震荡处理30min
七、裁取合适大小的尼龙膜或硝酸纤维素膜进行转膜操作,可用真空转膜仪或者搭滤纸桥,需要20×SSC
八、制备探针,可用地高辛标记(以地高辛为例)
九、将膜放入杂交瓶,42°C预杂交30min
十、倒掉预杂交液,加入杂交液,适当的温度进行杂交4h至过夜
十一、洗膜,先用2×SSC+0.1%SDS,20-25°C洗2×5min,再用0.5×SSC+0.1%SDS洗2×15min。然后用washing buffer洗1-5min,接着用blocking solution洗30min后,用antibody solution洗30min,再用washing buffer洗2×15min,再用detection buffer洗2-5min后,取出膜,放于保鲜膜上,在结合有DNA的一面滴加CSPD ready-to-use后,立刻盖上保鲜膜,让CSPD ready-to-use均匀的布满膜表面,室温放置5min后,37°C温育10min以上
十二、放射自显影,可用成像系统信号累积模式显影或用X-ray显影

