
Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1, kappa |
Purification : | Purified Ab with BSA and Azide at 200ug/ml |
Content : | 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Gene : | EPCAM |
Gene ID : | 4072 |
Uniprot ID : | P16422 |
Alternative Name : | Adenocarcinoma-associated Antigen,Cell Surface Glycoprotein Trop-1,EGP2,EGP314,EGP40,Epithelial Cell Adhesion Molecule,Epithelial Glycoprotein 314,ESA,KSA,TACD1,TROP1,Tumor-associated Calcium Signal Transducer 1 (TACSTD1),ECS-1,Epidermal Surface Antigen 1,ESA1,FLOT2,Flotillin-2,Membrane Component,Chromosome 17,Surface Marker-1 (M17S1),REG-1,Reggie-1,Reggie-2 |
Immunogen Information : | Neuraminidase treated GLS-1 human small cell lung carcinoma cells |
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Does not react with rat.
MW : 40-43kDa; Positive Control : HT29 cells or breast tumor;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1.0ug/ml); Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Lateral cell membrane, Cell junction |
Post transnational modification: | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
BioGrid: | 110250.5 interactions. |
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二、限制性内切酶消化样品DNA
三、凝胶电泳分离消化产物
四、如果靶序列>5kb,在0.25M的HCl中进行震荡脱嘌呤10min,ddH2O洗一次
五、用变性液震荡处理30min,ddH2O洗一次
六、中和液震荡处理30min
七、裁取合适大小的尼龙膜或硝酸纤维素膜进行转膜操作,可用真空转膜仪或者搭滤纸桥,需要20×SSC
八、制备探针,可用地高辛标记(以地高辛为例)
九、将膜放入杂交瓶,42°C预杂交30min
十、倒掉预杂交液,加入杂交液,适当的温度进行杂交4h至过夜
十一、洗膜,先用2×SSC+0.1%SDS,20-25°C洗2×5min,再用0.5×SSC+0.1%SDS洗2×15min。然后用washing buffer洗1-5min,接着用blocking solution洗30min后,用antibody solution洗30min,再用washing buffer洗2×15min,再用detection buffer洗2-5min后,取出膜,放于保鲜膜上,在结合有DNA的一面滴加CSPD ready-to-use后,立刻盖上保鲜膜,让CSPD ready-to-use均匀的布满膜表面,室温放置5min后,37°C温育10min以上
十二、放射自显影,可用成像系统信号累积模式显影或用X-ray显影

