| Product Name | Collagen (Type I), FITC conjugated *Water Soluble* |
| Size | 1 mg |
| Catalog # | AS-85111 |
| US$ | $267 |
AnaSpec offers two types of fluoresceinated collagen (type I). This product is water soluble and is used for quick fluorometric measurement of collagenase activity. In this protease substrate, collagen is heavily labeled with FITC, resulting in almost total quenching of the conjugate"s fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. This fluoresceinated collagen is useful for detecting MMP-1 activity.
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| Detailed Information | |
| Storage | -20°C desiccated and protected from light |
| References | Ref: Sela MN, et al. (2003). Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clin Oral Implants Res 14, 263-8; Baici A, et al. (1980). A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 108, 230-2; Sugiyama K, et al. (1980). A simple and rapid assay for collagenase activity using fluorescence-labeled substrate.Kurume Med J 27, 63-9. |
| Molecular Weight | N/D |
| Spectral Properties | Abs/Em = 492/515 nm |
| Solvent System | Water soluble |
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搞蛋白的:各类电泳设备,转膜仪,摇床,有需要还要特别搞一个暗房间
基本就这些吧,话说,兄台真要筹建一个实验室,为何不亲自到一个实验室转一圈呢?


