
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 141386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75* | Add to Cart | |
Lentiviral Prep | 141386-LV | Virus (1mL at titer ≥ 5x10⁶ TU/mL)and Plasmid.More Information | Add to Cart |
* Login to view industry pricing.
Backbone
- Vector backbonepLVX-EF1alpha-IRES-Puro(Search Vector Database)
- Backbone manufacturerTakara Bio
- Backbone sizew/o insert(bp)8825
- Vector typeMammalian Expression, Lentiviral
- Selectable markersPuromycin
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameSARS-CoV-2-M
- SpeciesSARS-CoV-2
- Mutationhuman codon optimized
- Entrez GeneM(a.k.a. GU280_gp05)
- PromoterEF1alpha
- Tag/ Fusion Protein
- 2xStrep (C terminal on insert)
Cloning Information
- Cloning methodUnknown
Resource Information
- Supplemental Documents
- pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro.gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Industry MTA
Information for Lentiviral Prep (Catalog # 141386-LV)(Back to top)
Purpose
Ready-to-use Lentiviral Prep particles produced from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro (#141386). In addition to the viral particles, you will also receive purified pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro plasmid DNA.
Lentiviral particles carrying SARS-CoV-2 membrane (M) protein. This virus is used to make stable cell lines expressing the SARS-CoV-2 membrane protein.Delivery
- Volume1mL
- Titer≥ 5x10⁶ TU/mL
- Pricing$100 USD for preparation of 1mL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging PlasmidspsPAX2 (plasmid #12260)
- EnvelopepMD2.G (plasmid #12259)
- BufferOptiPro
- Selectable MarkerPuromycin
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- ddPCR assay: 293T cells were transduced with serial dilutions of 141386-LV, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
- PCR confirmation of insert: PCR was carried out on the viral preparation with primers targeting the pLenti backbone and the insert. The PCR product was visualized on an agarose gel for size confirmation.
Visit our viral production page for moreinformation.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
谢谢!
主要是用于酵母,细菌等的转化.
t=RC,其中t是时程,R是电阻,C是电容,我不知道这个电阻是不是电转杯的电阻还是电转仪本身有个电阻,如果有哪位大侠知道能不能告诉?
联系方式:wuyixuansunny@sohu.com
表皮分布于幼嫩的根、茎、叶、花、果实和种子的表面,通常是一层由初生分生组织的原表皮分化而来的生活细胞(夹竹桃等植物的叶上表皮,具有多层生活细胞所组成的复表皮),是保护植株免于风、雨、病原微生物和虫害等伤害的初生保护组织。表皮以表皮细胞为主,另有保卫细胞、副卫细胞及外生物等,有些植物的表皮中还有特化的异细胞。表皮的形态特征是物种鉴定的依据之一。
POT -HG POT-LIN POT-LOG 三个都可调。其中POT-HG电阻上显示调整的%数这个估计需要详细的说明才弄的了去硬之城看看吧或许有人会。

