
Recombinant Human His6-UBE2F Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-679
Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5,200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2F
Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2) (1). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5 (1). Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING Ubiquitin Ligases (CRLs) (2). CRLs make up the largest subset of Ubiquitin Ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins (3).
- Huang, D.T. et al. (2009) Mol. Cell 33:483.
- Sarikas, A. et al. (2011) Genome Biol. 12:220.
- Duda, D.M. et al. (2011) Curr. Opin. Struct. Biol. 21:257.
FAQs
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而方波是一种数字信号,其电信号在一应时间内保持持续稳定的点击,并在点击后瞬间消失,对细胞的损伤较小,转化效率较高。
t=RC,其中t是时程,R是电阻,C是电容,我不知道这个电阻是不是电转杯的电阻还是电转仪本身有个电阻,如果有哪位大侠知道能不能告诉?
联系方式:wuyixuansunny@sohu.com
表皮分布于幼嫩的根、茎、叶、花、果实和种子的表面,通常是一层由初生分生组织的原表皮分化而来的生活细胞(夹竹桃等植物的叶上表皮,具有多层生活细胞所组成的复表皮),是保护植株免于风、雨、病原微生物和虫害等伤害的初生保护组织。表皮以表皮细胞为主,另有保卫细胞、副卫细胞及外生物等,有些植物的表皮中还有特化的异细胞。表皮的形态特征是物种鉴定的依据之一。

