Recombinant Human NEDD8 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-812
| Formulation | Xmg/ml (X μM) in 50 mM Hepes, pH 8, 150mM NaCl, and 1mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NEDD8
Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).
- 1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393. 2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
- 3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
- 4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
- 5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
- 6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
- 7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
- 8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
- 9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
- 10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
- 11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
- 12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Citations for Recombinant Human NEDD8 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
4Citations: Showing 1 - 4Filter your results:
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- Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein ExpressionAuthors: X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ DeshaiesMol. Cell, 2018;69(5):773-786.e6.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylationAuthors: H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S WangNat Commun, 2017;8(1):1150.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as afeedback regulator of biochemical and oncogenic activity.Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh BJ Biol Chem, 2015;290(1):296-309.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross JPLoS Pathog, 2012;8(12):e1003085.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
FAQs
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甲基硅油盐浴、磷酸浴、金属浴.十溴二苯乙烷 磷酸-三（2,3-二氯丙烷）酯1,2,3,4-四溴丁烷1,2-双（2,4,6-三溴苯氧基）乙烷
沥青:沸点(℃)：3000 °C (decomp)
La2O3:Boiling point 4200°C (4500 K)
Lu2O3:Boiling point 3980°C
ThO2:Boiling point 4400°C
要想买几个金属浴，没有特殊要求，只要体积小、请便易携就行。最好是国内主流牌子，样子要好看点的。进口的太贵了。
想H2O3系列的，天根什么的靠谱吗
第一步： 选择适用温度
了解样品最佳的实验温度，温度范围越大，相应仪器的价格也就越高，确认好了温度就可以进行下一步了。
第二步：选择试管加热类型
现有的加热类型主要分为两大类：
1 水浴：水浴最高温度在100℃
2 干浴(或称金属浴)：可选择的温度范围在100℃-250℃之间。
水浴优势：介质导热性好
水浴劣势：易污染
干式优势：洁净，使用方便
干式劣势：模块间存在极小的温差
第三步：选择模块
量一下需要使用的试管尺寸及使用数量，然后找到对应的模块。
氮吹仪就简单的选择好啦~~~
对于有更复杂功能的用户来说，只需要在提出自己的需求就好啦。
