DescriptionThe GF-1 AmbiClean Kit (Gel & PCR) is designed for rapid DNA recovery from agarose gel and PCR clean-up of DNA bands ranging from 100bp to 20kb. Special buffer provide the correct salt concentration and pH for efficient recovery (80-90%) of DNA from both PCR product and agarose gel from TAE or TBE buffers. The kit is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosphorylation, residual dye and ethidium bromide. This kit also allows for concentration of DNA, changing of buffers and desalting.
Features
- 90% of recovery achievable
- Purification process less than 15 minutes
- High pure DNA ready-to-use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, probe preparations, etc.
Kit Components
- Buffer DB
- Wash Buffer (concentrate)
- Elution Buffer
Ordering Information
Catalog No | Description | Pack Size |
GF-GC-050 | GF-1 AmbiClean Kit (Gel & PCR) | 50 preps |
GF-GC-100 | GF-1 AmbiClean Kit (Gel & PCR) | 100 preps |
GF-GC-200 | GF-1 AmbiClean Kit (Gel & PCR) | 200 preps |
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GF-1 AmbiClean Kit (Gel & PCR)
PublicationThis Product Has Been Used In: Al-Jallad et al. (2020)Molecular Characterization of Isolated Infectious Bronchitis Viruses from Affected Vaccinated Broiler Flocks in Syria, BMC Veterinary Research, 16:449.Bacus, MG et al. (2020) Global genetic patterns reveal host tropism versus cross-taxon 1 transmission of bat Betacoronaviruses, bioRxiv, https://doi.org/10.1101/2020.05.04.076281Donhatai Sruamsiri et al. (2020). In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast famine conditions,. Scientific Reports,10:3752.Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.Eshamah HL et al. (2020)Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.Elv Nhiel Salo & Annabelle Novero (2020) Identification and Characterisation of Endophytic Bacteria from Coconut (Cocos nucifera) Tissue Culture, Tropical Life Sciences Research,31(1), 2020.Insuk C, Kuncharoen N, Cheeptham N, Tanasupawat S & Pathom-aree W (2020)Bryophytes Harbor Cultivable Actinobacteria With Plant Growth Promoting Potential, Frontiers in Microbiology, 11:563047.Pimsiri Heepkaew & Benjaporn BS (2020) Polyhydroxyalkanoate production using two stage continuous stirred tank activated sludge systems with glycerol as a carbon source, Journal of Chemical Technology & Biotechnology, 95:1180-1190.Rahnama R et al. (2020)Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran, Iranian Journal of Fisheries Sciences, 19(3):1556-1572.Tamosiunalte A et al. (2020) What a Difference a Gene Makes: Identification of Virulence Factors of Cowpox Virus, Journal of Virology, 94:e01625-19.Luang-In et al. (2019) Protease-Producing Bacteria from Soil in Nasinuan Community Forest, Mahasarakham Province, Thailand, Biomedical and Pharmacology Journal, 12(2):587-595 Chong, S.Y., Rao, P.V. and Soon, Jan Mei (2017)Identification of Escherichia spp. strains in streetvended beverages and associated preparation surfaces using 16S rRNA analysis. International Food Research Journal, 24 (4). pp. 18111818Garbaj, A.M., Abolghait, S.K., Lawila, A.F., Azwai, S.M., Naas, H.T., Moawad, A.A., Gammoudi, F.T., Barbieri, I., Abureema, S., Eldaghayes, I. (2017). Molecular Identification, Prevalence and Antimicrobial Susceptibility Profile of Cronobacter spp. Cultivated on a Chromogenic Medium in Libya,Journal of Molecular Microbiology, Vol. 1, No. 1 (2017).Pasandideh, R., Nassiri, B., Shapouri, M.R.S.A., Fayazi, J., Roshanfeker, H., Lotfi, M (2017).Expression of the G1 Epitope of Bovine Ephemeral Fever Virus G Glycoprotein in Eukaryotic cells, Bulgarian Journal of Veterinary Medicine, 1311-1477 (2017). Said, M.B., Belkahia, H., Mabrouk,N.E., Saidani, M., Hassen, M.B., Alberti, A., Zobba, R., Bouattour, S., Bouattour, A., Messadi, L. (2017)Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia. Ticks and Tick-borne Diseases.8. Pp..412-422Said, M.B., Belhakia, H., Mabrouk, N.E., Saidani, M., Alberti ,A., Zobba,R., Cherif, A., Mahjoub, T., Bouattour, A., Messadi, L. (2017)Anaplasma platys-like strains in ruminants from Tunisia. Infection, Genetics and Evolution 49. Pp..226-233.Shamsi, H., Mardani, K., Ownagh, A. (2017). Phylogenetic analysis of Escherichia coli isolated from broilers with colibacillosis based on gyrA gene sequences. The Canadian Journal of Veterinary Research, Vol (81), 28-32 (2017).Azwai, S.M.et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya Open Veterinary Journal, 6(1), p. 36-43. Sriwichai, M., Malem, F., Pholchan, M.K., Bovonsombut, S. (2017).Detection of Bacterial Communities in Volatile-organic-compound (VOC)-contaminated Soil in an Industrial Estate in Eastern Thailand by PCR-DGGE Analysis, Chiang Mai Journal of Science, Vol. 44, No. 3, 742-750 (2017).Abadi, Z.S.N., Fatemi, F., Khosravani, M., Rahmati, M., Sadroddiny, E (2015).Molecular Cloning of Bone Morphogenetic Protein-2 (BMP- 2) in pGEM-b1 Vector and Transformation into E.coli Rosetta Strain, International Journal of Health Sciences, Vol. 1, No. 3 (2015).Belkahia, H., Said, M.B., Alberti, A., Abdi, K., Issaoui, Z., Hattab, D., Ghabi, M., Messadi, L. (2015) First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia. Infection, Genetics and Evolution. 34. Pp.361-371 Belkahia, H., Said, M.B., Sayahi, L., Alberti, A., Messadi, L. (2015). Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius), The Journal of Infection in Developing Countries, Vol. 9, No. 10, 1116-1125 Barghi, N., Concepcion, G. P., Olivera, B. M., Lluisma, A.O. (2015). Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes, Genome Biology and Evolution, Vol. 7, No. 6, 1797-1814 (2015).Deesuth, O., Laopaiboon, P., Klanrit, P., Laopaiboon, L. (2015) Improvement of ethanol production from sweet sorghum juice under high gravity and very high gravity conditions: Effects of nutrient supplementation and aeration. Industrial Crops and Products. 74. Pp.95-102.Emine, S., Zainol, N., Salihon, J., Convey, P (2015). Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi, International Journal of Extensive Research, No. 5, 79-87 (2015). Hamidinejat, H., et al. (2015)Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. OIran Journal of Parasitology. 10(1), p.69-77. Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015)Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490 Said, M.B., Belkahia, H., Karaoud, M., Bousrih, M., Yahiaoui, M. Daaloul-Jedidi, M., Messadi, L. (2015) First molecular survey of Anaplasma bovis in small ruminants from Tunisia.Veterinary Microbiology. 179. Pp.322-326.Seydametov, E (2015).Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil, International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 6, No. 2 (2015). Wiparat,S., Poeaim, S., Eiamampai, K., Atittayawan (2015). Gender Identification of Himantopus Himantopus Using PCR-Based Method, International Journal of Agricultural Technology, Chiang Mai Journal of Science, Vol. 11, No.2, 307-314 (2015).Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014)) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410. Mahboubi, M., Falsafi, T., Sadeghizadeh, M (2014). Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori, Iranian Journal of Biotechnology, Vol 12, No. 4, 10-16 (2014).Şakalar, Ç., Kuk, S., Erensoy, A., Dağli, A.F., Özercan, İ., Çetikaya, Ü, Yazar, S. (2014). Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus species, Turkish Journal of Medical Sciences, Vol. 44, 741-748 (2014). Xu, Z., Zikos, D., Tamošiunaite, A., Klopfleisch, R., Osterrieder, N., Tischer, B.K. (2014).Identification of 10 Coxpox Virus Proteins that are necessary for Induction of Hemorrhagic Lesions (Red Pocks) on Chorioallantoic Membranes,Vol. 88, No. 15 (2014). Nayabian, H., Mardani, K. (2013).Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran, Avian Pathology, Vol. 42, No. 2, 108-113 (2013). Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).
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1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!
pH(1)=pKa+lg[c(CH₃COONa)/c(CH₃COOH)]=pKa=4.74
通HCl后,溶液是c(CH₃COOH)=0.2mol/L、c(NaCl)=0.1mol/L的混合溶液,溶液pH按照弱酸溶液pH的求法求.
c(H⁺)=√[Ka*c(CH₃COOH)]=√(10^-4.74*0.2)=0.00191(mol/L)(采用了近似公式)
pH(2)=-lg{c(H⁺)}=2.72
两个pH求得,那么pH的变化量也就可得了.pH的变化量=|pH(2)-pH(1)|=|2.72-4.74|=2.02
1)PH缓冲溶液作用原理和pH值
当往某些溶液中加入一定量的酸和碱时,有阻碍溶液pH变化的作用,称为缓冲作用,这样的溶液叫做缓冲溶液.弱酸及其盐的混合溶液(如HAc与NaAc),弱碱及其盐的混合溶液(如NH3·H2O与NH4Cl)等都是缓冲溶液.
由弱酸HA及其盐NaA所组成的缓冲溶液对酸的缓冲作用,是由于溶液中存在足够量的碱A-的缘故.当向这种溶液中加入一定量的强酸时,H离子基本上被A-离子消耗:
所以溶液的pH值几乎不变;当加入一定量强碱时,溶液中存在的弱酸HA消耗OH-离子而阻碍pH的变化.
2)PH缓冲溶液的缓冲能力
在缓冲溶液中加入少量强酸或强碱,其溶液pH值变化不大,但若加入酸,碱的量多时,缓冲溶液就失去了它的缓冲作用.这说明它的缓冲能力是有一定限度的.
缓冲溶液的缓冲能力与组成缓冲溶液的组分浓度有关.0.1mol·L-1HAc和0.1mol·L-1NaAc组成的缓冲溶液,比0.01mol·L-1HAc和0.01mol·L-1NaAc的缓冲溶液缓冲能力大.关于这一点通过计算便可证实.但缓冲溶液组分的浓度不能太大,否则,不能忽视离子间的作用.
组成缓冲溶液的两组分的比值不为1∶1时,缓冲作用减小,缓冲能力降低,当c(盐)/c(酸)为1∶1时△pH最小,缓冲能力大.不论对于酸或碱都有较大的缓冲作用.缓冲溶液的pH值可用下式计算:
此时缓冲能力大.缓冲组分的比值离1∶1愈远,缓冲能力愈小,甚至不能起缓冲作用.对于任何缓冲体系,存在有效缓冲范围,这个范围大致在pKaφ(或pKbφ)两侧各一个pH单位之内.
弱酸及其盐(弱酸及其共轭碱)体系pH=pKaφ±1
弱碱及其盐(弱碱及其共轭酸)体系pOH=pKbφ±1
例如HAc的pKaφ为4.76,所以用HAc和NaAc适宜于配制pH为3.76~5.76的缓冲溶液,在这个范围内有较大的缓冲作用.配制pH=4.76的缓冲溶液时缓冲能力最大,此时(c(HAc)/c(NaAc)=1.
3)PH缓冲溶液的配制和应用
为了配制一定pH的缓冲溶液,首先选定一个弱酸,它的pKaφ尽可能接近所需配制的缓冲溶液的pH值,然后计算酸与碱的浓度比,根据此浓度比便可配制所需缓冲溶液.
以上主要以弱酸及其盐组成的缓冲溶液为例说明它的作用原理、pH计算和配制方法.对于弱碱及其盐组成的缓冲溶液可采用相同的方法.
PH缓冲溶液在物质分离和成分分析等方面应用广泛,如鉴定Mg2离子时,可用下面的反应:
白色磷酸铵镁沉淀溶于酸,故反应需在碱性溶液中进行,但碱性太强,可能生成白色Mg(OH)2沉淀,所以反应的pH值需控制在一定范围内,因此利用NH3·H2O和NH4Cl组成的缓冲溶液,保持溶液的pH值条件下,进行上述反应.
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?