HumanPrimaryLungMicrovascularEndothelialCells
CatalogNo. H-6011
SuggestedMedium: CompleteHumanEndothelialCellMedium–500ML
CatalogNo. H1168
ProductDescription
HumanPrimaryLungMicrovascularEndothelialCellsfromCellBIOLOGicsareisolatedfromhumanlungtissue.HumanPrimaryLungMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryopreservedinvails.Eachvialcontainsatleast0.5×106 cellsperml. Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
HumanPrimaryLungMicrovascularEndothelialCellsfromCellBiologicsdisplaytypicalcobblestonewithlargedarknucleiappearanceunderlightmicroscopy.CellsaretestedforexpressionofendothelialcellMarkerusingantibody,CD31(CatalogNo.550389,BD;CD31/PECAM-1PE-conjugatedAntibody,CatalogNo.FAB3567P,R&D)orVE-Cadherin(FITC-VE-cadherinCatalogNo.560411,BD)byimmunofluorescencestainingorFACS.Allcellstestnegativeformycoplasma,bacteria,yeast,andfungi.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.Perrequest,aCertificateofAnalysiswillbeprovidedforeachcelllotpurchased.Cellscanbeexpandedfor3-5passagesunderthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
HumanPrimaryLungMicrovascularEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
HumanPrimaryLungMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.
CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughHumanPrimaryLungMicrovascularEndothelialCellsareisolatedfromnormalhumantissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ humanendothelialcellswith CellBiologics’ Media(catalognumberH1168) andCoatingSolutionforappropriateendothelialcellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
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