ProTx-III,newselectiveNav1.7blocker
ProTx-III (µ-TRTX-Tp1a;belongstoNaSpTxfamily1)hasbeenisolatedfromthevenomofthePeruviangreen-velvettarantulaThrixopelmapruriensattheUniversityofQueensland(Australia).ProTx-IIIhasbeendescribedtobeaselectiveinhibitorofNav1.7withanIC50valuearound2nM.ThesyntheticversionofTp1aproducedbySmartoxyieldssimilarresultsintermsofNav1.7inhibition(seeFigure1).QueenslandscientistshavedescribedthatProTx-III inducesnosignificantchangesinthevoltagedependenceofactivationorsteady-stateinactivationofNav1.7.ProTx-III alsoinhibitsNav1.1,Nav1.2 andNav1.6atnanomolarconcentrations anddoesnotaffectsCavchannelsandnicotinicacetylcholinereceptors. ProTx-IIIdemonstratesanalgesicpropertiesinvivowheninjectedintraplantaryinmicepriortreatmentwithOD1,anactivatorofNav1.7,whichinducesspontaneouspain.
Fig1:effectof5nMSmartoxProTx-IIIonhNav1.7current studiedbyelicitingvoltagestepsfrom-60to+40mVataholdingpotentialof-90mV.
Viewbioassayreport
Description:
AAsequence: Asp-Cys2-Leu-Lys-Phe-Gly-Trp-Lys-Cys9-Asn-Pro-Arg-Asn-Asp-Lys-Cys16-Cys17-Ser-Gly-Leu-Lys-Cys22-Gly-Ser-Asn-His-Asn-Trp-Cys29-Lys-Leu-His-Ile-NH2
Disulfidebonds: Cys2-Cys17,Cys9-Cys22,Cys16-Cys29
Length(aa):33
Formula: C162H246N52O43S6
Appearance:Whitelyophilizedsolid
CASnumber:
MolecularWeight:3802.47Da
Source:Synthetic
Solubility:Waterorsalinebuffer,5mg/mLmaximum(recommendation)
Counterion:TFAsalts
Reference:
IdentificationandCharacterizationofProTx-III
Spidervenomsarearichsourceofionchannelmodulatorswiththerapeuticpotential.Giventheanalgesicpotentialofsubtype-selectiveinhibitorsofvoltage-gatedsodium(NaV)channels,wescreenedspidervenomsforinhibitorsofhumanNaV1.7(hNaV1.7)usingahigh-throughputfluorescentassay.Here,wedescribethediscoveryofanovelNaV1.7inhibitor,μ-TRTX-Tp1a(Tp1a),isolatedfromthevenomofthePeruviangreen-velvettarantulaThrixopelmapruriens.Recombinantandsyntheticformsofthis33-residuepeptidepreferentiallyinhibitedhNaV1.7>hNaV1.6>hNaV1.2>hNaV1.1>hNaV1.3channelsinfluorescentassays.NaV1.7inhibitionwasdiminished(IC5011.5nM)andtheassociationratedecreasedfortheC-terminalacidformofTp1acomparedwiththenativeamidatedform(IC502.1nM),suggestingthatthepeptideCterminuscontributestoitsinteractionwithhNaV1.7.Tp1ahadnoeffectonhumanvoltage-gatedcalciumchannelsornicotinicacetylcholinereceptorsat5μM.UnlikemostspidertoxinsthatmodulateNaVchannels,Tp1ainhibitedhNaV1.7withoutsignificantlyalteringthevoltagedependenceofactivationorinactivation.Tp1aprovedtobeanalgesicbyreversingspontaneouspaininducedinmicebyintraplantarinjectioninOD1,ascorpiontoxinthatpotentiateshNaV1.7.ThestructureofTp1aasdeterminedusingNMRspectroscopyrevealedaclassicinhibitorcystineknot(ICK)motif.ThemolecularsurfaceofTp1apresentsahydrophobicpatchsurroundedbypositivelychargedresidues,withsubtledifferencesfromotherICKspidertoxinsthatmightcontributetoitsdifferentpharmacologicalprofile.Tp1amayhelpguidethedevelopmentofmoreselectiveandpotenthNaV1.7inhibitorsfortreatmentofchronicpain.
FernandaC.,etal.(2015)IdentificationandCharacterizationofProTx-III[m-TRTX-Tp1a],aNewVoltage-GatedSodiumChannelInhibitorfromVenomoftheTarantulaThrixopelmapruriens.PMID: 25979003
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
常用流动相加酸碱后PH的总结，希望大家能够提供一点自己测过的结果，谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液，再调pH到7.4；（我们试着用这个做了下，发现挂不上柱）
2.配置磷酸钠盐缓冲液：按NaH2PO4：Na2HPO4以19：81的摩尔比配制成pH7.4的缓冲液？（附一张百度出来的配方
）
3.如果是磷酸钠盐缓冲液，可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗？
再者，2和3这两个方法配制的磷酸钠盐缓冲液有什么区别？最终效果是一样的吗？如果不一样，有什么理论的知识支撑呢？个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外，我还想问一下，pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗？是怎么影响的？谢谢大家了！
有了源数据之后把源数据按照大小排列，
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响，样品又不好改变其PH值，这种情况怎么办？希望有经验的高手指教。
我的流动相是甲醇-水（90：10）
谢谢赐教！
请进子版按格式发贴，自行修改，谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液，能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响，从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6？能否直接用pH计测量？谢谢!
暂无品牌分类
© 2005- 苏州蚂蚁淘生物科技有限公司 版权所有。
苏ICP备17049038号-11
苏公网安备 32059002001627号
声明：本网站部分产品受外界条件影响（包含但不限于进口国出口国政策限制等因素），无法提供销售服务，下单后，会由客服人员联系厂家确认后另行通知。部分危险品无法清关，请勿下单