
Ready-to-Use | Fine-gel SDS-PAGE Gel Solution is a TEMED free, ready-to-pour premixed solution of acrylamide, bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE |
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No TEMED | This novel formulation requires only the addition of APS |
Gradient Gel Like Seperation | This novel formulation allows separation of a wide range of proteins from 10kDa to 240 kDa on the same mini-gel with less prep work than traditional SDS-PAGE. |
Application | SDS-PAGE Gel |
Fine-gel SDS-PAGE Gel Solution(15%) is a TEMED free, ready-to-pour premixed solution of acrylamide,bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE. This novel formulation requires only the addition of APS and allows separation of proteins from 2kDa to 100 kDa on the same mini-gel with less prep work than traditional SDS-PAGE.
This product provides a superior resolution and a significant time savings, with no need to weigh hazardous acrylamide powder or pour stacking gel separately. The gel is compatible with post-electrophoresis applications such as Western blotting, MALDI analysis, protein sequencing and other downstream applications. It is also suitable for staining with all commonly used dyes such as Coomassie Brilliant Blue, silver stain and fluorescent dyes.
The Fine-gel SDS-PAGE Runnung Buffer(10X) is a unique, optimized running buffer to provide enhanced resolution and decrease running-time compared to traditional gel running buffer. The Fine-gel SDS-PAGE Runnung Buffer(10X) is fully compatible with other general acrylamide solution.
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但是最好还是不要用,影响实验就不好了。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

