| Inhibited Protease | Serine, Cysteine, Aspartic, and Metalloprotease |
|---|---|
| Downstream App | No interfering such as 2-D gel and His-tagged protein purification |
| EDTA Free | 0.5M EDTA solution supplied in a seperate vial |
The Xpert Protease Inhibitor Cocktail Solution(100X) is
a specially formulated mixture of 5 protease inhibitors
with broad specificity for the inhibition of various protease
and esterase. Other EDTA-free inhibitor cocktails only
provide protection against serine and cysteine protease.
The Xpert Protease inhibitor cocktail solution have
a proprietary form of pepstatin A and Bestatin that allow
for additional protection against aspartic and
metalloprotease without interfering with downstream
applications, such as 2-D gel and His-tagged protein
purifications. It is provided as either a set of 5,10 vials
or as a single vial with 0.5M EDTA.
The Xpert Protease Inhibitor Cocktail Solution(100X) is
ready-to-use 100X stock solution and does not require
reconstitution with water or solvent.
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

