
Ready-to-Use | Fine-gel SDS-PAGE Gel Solution is a TEMED free, ready-to-pour premixed solution of acrylamide, bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE |
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No TEMED | This novel formulation requires only the addition of APS |
Gradient Gel Like Seperation | This novel formulation allows separation of a wide range of proteins from 10kDa to 240 kDa on the same mini-gel with less prep work than traditional SDS-PAGE. |
Application | SDS-PAGE Gel |
Fine-gel SDS-PAGE Gel Solution(15%) is a TEMED free, ready-to-pour premixed solution of acrylamide,bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE. This novel formulation requires only the addition of APS and allows separation of proteins from 2kDa to 100 kDa on the same mini-gel with less prep work than traditional SDS-PAGE.
This product provides a superior resolution and a significant time savings, with no need to weigh hazardous acrylamide powder or pour stacking gel separately. The gel is compatible with post-electrophoresis applications such as Western blotting, MALDI analysis, protein sequencing and other downstream applications. It is also suitable for staining with all commonly used dyes such as Coomassie Brilliant Blue, silver stain and fluorescent dyes.
The Fine-gel SDS-PAGE Runnung Buffer(10X) is a unique, optimized running buffer to provide enhanced resolution and decrease running-time compared to traditional gel running buffer. The Fine-gel SDS-PAGE Runnung Buffer(10X) is fully compatible with other general acrylamide solution.
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低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧

