
Ready-to-Use | Fine-gel SDS-PAGE Gel Solution is a TEMED free, ready-to-pour premixed solution of acrylamide, bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE |
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No TEMED | This novel formulation requires only the addition of APS |
Gradient Gel Like Seperation | This novel formulation allows separation of a wide range of proteins from 10kDa to 240 kDa on the same mini-gel with less prep work than traditional SDS-PAGE. |
Application | SDS-PAGE Gel |
Fine-gel SDS-PAGE Gel Solution(15%) is a TEMED free, ready-to-pour premixed solution of acrylamide,bisacrylamide, buffer, and SDS that enables ultra-fine resolution of protein bands by denaturing PAGE. This novel formulation requires only the addition of APS and allows separation of proteins from 2kDa to 100 kDa on the same mini-gel with less prep work than traditional SDS-PAGE.
This product provides a superior resolution and a significant time savings, with no need to weigh hazardous acrylamide powder or pour stacking gel separately. The gel is compatible with post-electrophoresis applications such as Western blotting, MALDI analysis, protein sequencing and other downstream applications. It is also suitable for staining with all commonly used dyes such as Coomassie Brilliant Blue, silver stain and fluorescent dyes.
The Fine-gel SDS-PAGE Runnung Buffer(10X) is a unique, optimized running buffer to provide enhanced resolution and decrease running-time compared to traditional gel running buffer. The Fine-gel SDS-PAGE Runnung Buffer(10X) is fully compatible with other general acrylamide solution.
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此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。

