M.Wt: 405.27Formula: C14H24N2O7.2HClSolubility: UnknownPurity: >99%Storage: at -20? 2 yearsCAS No.: 21736-83-4
White to off-white solid. A broad spectrum aminoglycoside antibiotic that contains two glucose moieties. Footprint studies indicate that spectinomycin exerts regional effects on ribosomal structure. A resistance gene (aph) which encodes a spectinomycin has been cloned from Legionella pneumophila (disease agent for Legionnaire’s disease) kinase and expressed in E. coli. Potency: ≥500 µg/mg. Soluble in H2O. RTECS WG7400000, CAS 22189-32-8, M.W. 495.4. References: Merck Index 13, 8813.Thompson, P.R., et al. 1998. J. Biol. Chem. 273, 14788.Noah, J., and Wollenzien, P. 1997. Nucleic Acids Symp. Ser. 36, 178.Suter, T.M., et al. 1997. Antimicrob. Agents Chemother. 41, 1385.Thom, G., and Prescott, C.D. 1997. Bioorg. Med. Chem. 5, 1081.
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

