BSA, Biotechnology grade, 100g.Bovine serum albumin (BSA) is a large globular protein (66,000 Dal) with a good essential amino acid profile .It has been well characterized and the physical properties of this protein are wellknown ( Peters, 1975). BSA binds free fatty acids, other lipids and flavor compounds, which can alter the heat denaturation of the protein (Kinsella and Whitehead, 1992).Isolated BSA has been reported to be a very functional protein (Waniska, et.al., 1981).It is reported to partially unfold between 40 and 50oC, exposing non-polar residues on the surface and facilitating reversible protein-protein interactions.
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

