BSA, Biotechnology grade, 100g.Bovine serum albumin (BSA) is a large globular protein (66,000 Dal) with a good essential amino acid profile .It has been well characterized and the physical properties of this protein are wellknown ( Peters, 1975). BSA binds free fatty acids, other lipids and flavor compounds, which can alter the heat denaturation of the protein (Kinsella and Whitehead, 1992).Isolated BSA has been reported to be a very functional protein (Waniska, et.al., 1981).It is reported to partially unfold between 40 and 50oC, exposing non-polar residues on the surface and facilitating reversible protein-protein interactions.
ebiomall.com
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低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
但是最好还是不要用,影响实验就不好了。
如果你是制胶或者做电泳缓冲液的话,不用灭菌。

