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Tissue-Tearor
The Tissue-Tearor is a rotor/stator type tissue homogenizer which can rapidly homogenize, disrupt, and emulsify plant and animal samples in 0.5 - 1000 ml of liquid. Complete homogenization of tissues (muscle, liver, breast tissue, etc.) is usually achieved in ten to thirty seconds. Little, if any, heat is produced during the process. Rotor/stator homogenizers cannot efficiently disrupt microorganisms. This "limitation" can be an advantage if you want to homogenize or disperse tissue sample (or soil or waste samples) in order to recover viable bacteria or viruses for plating and analysis.
If you need a 230 volt unit, please select models that end in EUR. When ordering select one of our four different probe sizes - 04, 07, XL, and 14. Unlike most competitive Brands of rotor-stator homogenizer, THE TISSUE-TEAROR COMES COMPLETE WITH BOTH THE HIGH-SPEED MOTOR AND ONE STAINLESS STEEL PROBE OF YOUR CHOICE. See chart below for probe selection guidance. Additional probes are available. All are interchangeable with the TT motor unit. A cord-less variety of the TissueTearor is also available with model numbers ending in CL.
Our Price : $0.00
The Tissue-Tearor comes complete with a high speed, variable speed electric motor, one probe of your choice and a plastic storage stand. All probes are interchangeable with the Tissue-Tearor motor. See Parts & Acc's to order additional probes alone. The Tissue-Tearor with the 14 mm diameter probe does not come with a plastic storage stand.
Catalog Number | Practical Working Range (ml) | Probe Diameter (mm) | Probe Length (cm) |
985370-04 | 0.2-5.0 | 4.5 | 4.8 |
985370-07 | 0.5-50 | 7 | 8.3 |
985370-14 | 5.0-1000 | 14 | 13.2 |
985370-XL | 0.5-50 | 7 | 10.9 |
* Dimensions taken from stator.
Scents | Apricot, Lemon & Mango |
Color | test |
- Sample Volume Range: 0.2 to 1000 ml, depending on the probe selected
- Universal Motor: 110-120 volt, AC, 1.2 amp. Or, for some overseas applications, 230 volt, AC
- Probes: Type 316 stainless steel with fluorocarbon bushings
- Weight: Approximately 1/2 Kg
- Use for tissue cell disruption, emulsification and extreme mixing
- Practical answer for small samples of 10 mg to a few grams
- Easy to hold and light weight for one hand operation. Can also be stand mounted
- Inert to aggressive solutions. Stainless steel probe easily cleaned when processing multiple samples
- All probes are interchangeable and can be removed for autoclaving
- Extremely high speed 32,000 rpm motor with variable speed control
AWAY FROM ELECTRICAL LINES? Consider the model 780CL Cordless Tissue-Tearor. The purchase includes a cordless (i.e., battery operated), high speed motor, one probe of your choice, a rechargeable 9.6 volt Li battery pack and a plug-in battery charger. The maximum speed of the 780CL is slightly slower than the line-operated TissueTearor (25,000 vs 35,000 rpm). However, 25,000 rpm is quite adequate for homogenizing soft tissue like muscle, liver, or brain. The price for the 780CL Tissue-Tearor is the same as the line powered 985370 series. To order a Cordless Tissue-Tearor, combine the 780CL model number with the probe of your choice using the above table (example: 780CL-07).
OPERATING INSTRUCTIONS. See http://www.biospec.com/instructions/tissue_tearor/.
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TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢?
比如如果是Invitrogen的预制胶-预混液电泳系统,每种预制胶有适配缓冲液,但是如果用其他的缓冲液似乎也可以得到不错的结果,那么是否说明电泳缓冲液只要满足了缓冲ph范围,其他的不是特别重要呢?
此外,附加提问是,PVDF膜在转膜的时候,转膜缓冲液中不需要加入甲醇,那么是加入甲醇转膜效果好还是不加好呢?
谢谢!
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