Tissue-Tearor
The Tissue-Tearor is a rotor/stator type tissue homogenizer which can rapidly homogenize, disrupt, and emulsify plant and animal samples in 0.5 - 1000 ml of liquid. Complete homogenization of tissues (muscle, liver, breast tissue, etc.) is usually achieved in ten to thirty seconds. Little, if any, heat is produced during the process. Rotor/stator homogenizers cannot efficiently disrupt microorganisms. This "limitation" can be an advantage if you want to homogenize or disperse tissue sample (or soil or waste samples) in order to recover viable bacteria or viruses for plating and analysis.
If you need a 230 volt unit, please select models that end in EUR. When ordering select one of our four different probe sizes - 04, 07, XL, and 14. Unlike most competitive Brands of rotor-stator homogenizer, THE TISSUE-TEAROR COMES COMPLETE WITH BOTH THE HIGH-SPEED MOTOR AND ONE STAINLESS STEEL PROBE OF YOUR CHOICE. See chart below for probe selection guidance. Additional probes are available. All are interchangeable with the TT motor unit. A cord-less variety of the TissueTearor is also available with model numbers ending in CL.
Our Price : $0.00
The Tissue-Tearor comes complete with a high speed, variable speed electric motor, one probe of your choice and a plastic storage stand. All probes are interchangeable with the Tissue-Tearor motor. See Parts & Acc's to order additional probes alone. The Tissue-Tearor with the 14 mm diameter probe does not come with a plastic storage stand.
| Catalog Number | Practical Working Range (ml) | Probe Diameter (mm) | Probe Length (cm) |
| 985370-04 | 0.2-5.0 | 4.5 | 4.8 |
| 985370-07 | 0.5-50 | 7 | 8.3 |
| 985370-14 | 5.0-1000 | 14 | 13.2 |
| 985370-XL | 0.5-50 | 7 | 10.9 |
* Dimensions taken from stator.
| Scents | Apricot, Lemon & Mango |
| Color | test |
- Sample Volume Range: 0.2 to 1000 ml, depending on the probe selected
- Universal Motor: 110-120 volt, AC, 1.2 amp. Or, for some overseas applications, 230 volt, AC
- Probes: Type 316 stainless steel with fluorocarbon bushings
- Weight: Approximately 1/2 Kg
- Use for tissue cell disruption, emulsification and extreme mixing
- Practical answer for small samples of 10 mg to a few grams
- Easy to hold and light weight for one hand operation. Can also be stand mounted
- Inert to aggressive solutions. Stainless steel probe easily cleaned when processing multiple samples
- All probes are interchangeable and can be removed for autoclaving
- Extremely high speed 32,000 rpm motor with variable speed control
AWAY FROM ELECTRICAL LINES? Consider the model 780CL Cordless Tissue-Tearor. The purchase includes a cordless (i.e., battery operated), high speed motor, one probe of your choice, a rechargeable 9.6 volt Li battery pack and a plug-in battery charger. The maximum speed of the 780CL is slightly slower than the line-operated TissueTearor (25,000 vs 35,000 rpm). However, 25,000 rpm is quite adequate for homogenizing soft tissue like muscle, liver, or brain. The price for the 780CL Tissue-Tearor is the same as the line powered 985370 series. To order a Cordless Tissue-Tearor, combine the 780CL model number with the probe of your choice using the above table (example: 780CL-07).
OPERATING INSTRUCTIONS. See http://www.biospec.com/instructions/tissue_tearor/.
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BioHomogenizer
The BioHomogenizer is a rotor-stator type tissue homogenizer designed to rapidly homogenize, disperse, and emulsify biological samples in aqueous media from 1 to 1000 ml. The BioHomogenizer includes both the motor and probe.
Cat. No. 1281, BioHomogenizer for 5-200ml samples, complete with 1.4 cm probeCat. No. 1281EUR, 230 volt version of No. 1281Cat. No. 1285, BioHomogenizer for 100-2000ml samples, complete with 2.5 cm probeCat. No. 1285EUR, 230 volt version of No. 1285
Read more >Price: $830.00
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② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大，甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高，也是一个原因
我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) ：将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中，加水至终体积为500ml
使用时稀释至1×的缓冲液，电泳槽内槽加入阴极电泳缓冲液，外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
如果你是制胶或者做电泳缓冲液的话，不用灭菌。
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢？
比如如果是Invitrogen的预制胶-预混液电泳系统，每种预制胶有适配缓冲液，但是如果用其他的缓冲液似乎也可以得到不错的结果，那么是否说明电泳缓冲液只要满足了缓冲ph范围，其他的不是特别重要呢？
此外，附加提问是，PVDF膜在转膜的时候，转膜缓冲液中不需要加入甲醇，那么是加入甲醇转膜效果好还是不加好呢？
谢谢！
