
Effect Diluent Medium
Sample Diluent & RF/HAMA Blocker
Cat. No. 5080
The Effect Diluent is an effective buffer for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. It efficiently minimizes cross-reactions and unspecific binding in immunoassays like ELISA, EIA, RIA, Western Blotting, Immuno-PCR, Protein arrays, multianalyte immunoassays and Immunohistochemistry.The Effect Diluent is used alternatively to the standard sample or antibody dilution buffer:
- In ELISA for the dilution of specimen and detection antibodies.
- In Western Blotting for the dilution of primary and secondary antibodies.
- In IHC for the dilution of primary and secondary antibodies.
- In Protein arrays for the dilution of specimen and detection antibodies.
Three versions of the Effect Diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g. by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.
This product is not for sale in the US.
Specifications
- Available sizes:500 mL
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C
- Stability:3 years
Downloads
Download your LOT specific Certificate of Analysis (CoA)
ebiomall.com






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我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.

