Description
MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.
MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang, resulting in PCR product suitable for effective integration into TA cloning vectors.
Product Highlights
- Direct gel loading - no need for further post-PCR processing steps
- Easy visual recognition- reduces pipetting errors
- Robust performance - perfect for a wide range of PCR reactions
- Reproducible results - consistent QC ensures reliability
Product Description
MangoTaq™ DNA Polymerase offers high yield across a wide range of DNA concentrations. MangoTaq DNA Polymerase leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
The polymerase is supplied with two different reaction buffers for greater flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.
The two reaction buffers supplied are: 5x Colored Reaction Buffer and 5x Colorless Reaction Buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although extremely rare exceptions may exist.
Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection. The specificity and performance of MangoTaq DNA Polymerase can be further improved with the use of 3% DMSO, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
Applications
- High throughput applications
- Suited to a wide range of PCR assays
- Products suitable for TA cloning
- Direct loading
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchApplication Note
MangoTaq™ DNA Polymeraseebiomall.com
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② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大，甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高，也是一个原因
