Description
MangoMix™ is a convenient, ready-to-go, 2x Reaction Mix containing MangoTaq™ DNA Polymerase, MgCl2 and ultra-pure dNTPs manufactured by Meridian. The Mix is optimized and ready-to-use and requires only the addition of water, template and primers.
Product Highlights
- Direct gel loading - no need for further post-PCR processing steps
- Easy visual recognition- reduces pipetting errors
- High performance - pre-optimized 2x solutions
- Ready to use format - reduces risk of contamination and decreases the reaction set-up time
- Reproducible results - consistent QC ensures reliability
Product Description
MangoMix™ is optimized and ready-to-use, so the user need only add water, template and primers. MangoMix reduces the time required to set up reactions, thereby minimizing contamination risks and providing greater reproducibility through a reduction in the number of pipetting steps. MangoMix can be loaded directly onto an agarose gel for analysis, without the need for a separate gel-loading buffer.
The presence of dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist. MangoMix has been optimized for a wide variety of templates. An additional 50 mM of MgCl2 solution is included should any fine adjustments be required.
Applications
- High throughput applications
- Suited to a wide range of PCR assays
- Products suitable for TA cloning
- Direct loading

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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

