Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
U-120
| Formulation | Lyophilized from a solution in deionized water. |
| Reconstitution | Reconstitute at 10 mg/mL in an aqueous solution. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Ubiquitin
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
This fully functional N-terminal FLAG-tagged Ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This tag is specifically recognized by Anti-FLAG antibodies M1, M2 or M5.
- Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
- Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
- Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Wertz, I.E. et al. (2004) Nature 430:694.
Citations for Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
13Citations: Showing 1 - 10Filter your results:
Filter by:
- Ubiquitination of MAP1LC3B by pVHL is associated with autophagy and cell death in renal cell carcinomaAuthors: HM Kang, KH Noh, TK Chang, D Park, HS Cho, JH Lim, CR JungCell Death Dis, 2019;10(4):279.Applications: Bioassay
- Ring finger protein 145 (RNF145) is a ubiquitin ligase for sterol-induced degradation of HMG-CoA reductaseAuthors: LY Jiang, W Jiang, N Tian, YN Xiong, J Liu, J Wei, KY Wu, J Luo, XJ Shi, BL SongJ. Biol. Chem., 2018;0(0):.Applications: Bioassay
- Proteasome-associated HECT-type ubiquitin ligase activity is required for plant immunityAuthors: JJ Furniss, H Grey, Z Wang, M Nomoto, L Jackson, Y Tada, SH SpoelPLoS Pathog., 2018;14(11):e1007447.Species: HumanSample Types: ProteinApplications: Bioassay
- The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor TurnoverAuthors: R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T HoppeCell, 2017;169(3):470-482.e13.Applications: Bioassay
- Reciprocal Regulation between 53BP1 and the Anaphase-Promoting Complex/Cyclosome Is Required for Genomic Stability during Mitotic StressAuthors: TJ Kucharski, PE Minshall, M Moustafa-K, AS Turnell, JG TeodoroCell Rep, 2017;18(8):1982-1995.
- Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chainsAuthors: C Liu, W Liu, Y Ye, W LiNat Commun, 2017;8(0):14274.Species: YeastSample Types: Cell LysatesApplications: Bioassay
- COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongationAuthors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T MaoProc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP�NPLOC4�UFD1L is enhanced by a mutation that causes multisystem proteinopathyAuthors: EE Blythe, KC Olson, V Chau, RJ DeshaiesProc. Natl. Acad. Sci. U.S.A., 2017;0(0):.Applications: Bioassay
- Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1Elife, 2016;5(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination
- Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, WeiEMBO J, 2014;33(1):46-61.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity.Authors: Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph SJ Virol, 2014;88(11):6411-22.Species: N/ASample Types: ProteinApplications: Bioassay
- Cytosolic quality control of mislocalized proteins requires RNF126 recruitment toBag6.Authors: Rodrigo-Brenni M, Gutierrez E, Hegde RMol Cell, 2014;55(2):227-37.Applications: Bioassay
- An unusual two-step control of CPEB destruction by Pin1.Authors: Nechama, Morris, Lin, Chien-Li, Richter, Joel DMol Cell Biol, 2013;33(1):48-58.Species: XenopusSample Types: In VivoApplications: In Vivo
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReconstitution Buffers
Reconstitution Buffer 1 (PBS)
Reviews for Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF and earn rewards!
Have you used Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
低离子强度时，迁移率快。但离子强度过低，缓冲液的缓冲容量小，不易维持pH恒定。高离子强度时迁移率慢，因此为了获得更快的反应速度，在缓冲容量允许的范围内，离子强度应该尽可能小。
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
② 应取pH8.0，这样可使核苷酸带较多负电荷，利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷，但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时，根据核苷酸负电荷的多少来决定洗脱速度，则洗脱顺序为CMP>AMP> GMP > UMP，但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附，嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为：CMP> AMP > UMP >GMP。
