| Product Name | MOG (92 - 106)DEGGYTCFFRDHSYQ |
| Size | 1 mg |
| Catalog # | AS-62732 |
| US$ | $96 |
| Purity | % Peak Area By HPLC ≥ 95% |
This is amino acids 92 to 106 fragment of the myelin oligodendrocyte glycoprotein (MOG). Mice with MOG (92106)-induced experimental autoimmune encephalomyelitis develop extensive B cell reactivity against secondary myelin antigens. Despite the fact that this MOG peptide induces only weak T cell responses, MOG-induced autoimmunity is very severe. This peptide is encephalitogenic in SJL mice, DA rats, and rhesus monkeys. | |
| Detailed Information | |
| Storage | -20°C |
| References | Bischof, F. et al. J. Immunol. 173, 600 (2004). |
| Molecular Weight | 1824.9 |
| DEGGYTCFFRDHSYQ | |
| Sequence(Three-Letter Code) | H - Asp - Glu - Gly - Gly - Tyr - Thr - Cys - Phe - Phe - Arg - Asp -His - Ser - Tyr - Gln - OH |
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

