
- SpeciesReactivityHuman
- SpecificityDetectshumanTGF-beta RIIIindirectELISAsandWesternblots.IndirectELISAs,approximately25%cross-reactivitywithrecombinantmouse(rm)TGF-beta RIIIisobserved,andlessthan1% cross-reactivitywithrecombinanthuman(rh)TGF-beta RI,rhTGF-beta RII,rhTGF-beta RIIB,andrmTGF-beta RI isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanTGF‑beta RIII
Gly21-Asp781
Accession#AAA67061 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelFluorescein
- FlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- LongName:TransformingGrowthFactorbetaReceptorIII
- EntrezGeneIDs:7049(Human);21814(Mouse);29610(Rat)
- AlternateNames:betaglycanproteoglycan;Betaglycan;BGCAN;TBRIII;TGF-betareceptortype3;TGF-betareceptortypeIII;TGF-betaRIII;TGFbetaRIII;TGFBR3;TGF-bRIII;TGFR-3;TransforminggrowthfactorbetareceptorIII;transforminggrowthfactorbetareceptortype3;transforminggrowthfactor,betareceptorIII(betaglycan,300kDa);transforminggrowthfactor,betareceptorIII
Background:
MostcelltypesexpressthreesizesofreceptorsforTGF-beta.ThesearedesignatedTypeI(53kDa),Type II(70-85kDa),andTypeIII(250-350kDa).TheTypeIreceptorisamembrane-boundserine/threoninekinasethatapparentlyrequiresthepresenceoftheTypeIIreceptortobindTGF-beta.TheTypeIIreceptorisalsoamembrane-boundserine/threoninekinasethatbindsTGF-beta1andTGF-beta3withhighaffinityandTGF-beta2withamuchloweraffinity.TheTypeIandTypeIIreceptorstogetherformaheterodimericsignalingcomplexthatisessentialforthetransductionoftheanti-proliferativesignalsofTGF-beta.
TheTypeIIIreceptorisatransmembraneproteoglycanwithalargeextracellulardomainanda43aminoacidresiduecytoplasmicdomain.ThecytoplasmicdomainoftheTypeIIIreceptorlacksanobvioussignalingmotifandthereceptormaynotbeinvolveddirectlyinsignaltransduction.TheTypeIIIreceptorbindsTGF-beta2withthehighestaffinity.OtherTGF-betaisoformsalsobindtheTypeIIIreceptor,butwithloweraffinities.CellularresponsivenesstoTGF-beta2appearstobedependentonthepresenceoftheTypeIIIreceptorwhichcaninteractwiththesignalingreceptorcomplex.InadditiontothetransmembraneTypeIIIreceptor,asolubleformofthereceptorissecretedbysomecelltypes.Thephysiologicalroleofthissolublereceptorremainstobedetermined.TherecombinantTGF-betasolublereceptorTypeIIIbindstheTGF-betaisoformsdifferentiallyinsolutionandexhibitsTGF-betaantagoNISTicactivitiesinvitro.
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我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因


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