
- SpeciesReactivityHuman
- SpecificityDetectshumanCXCL9/MIGinELISAsandWesternblots. InELISAs,doesnotcross-reactwithrecombinantmouse(rm) CXCL9,recombinanthumanCXCL10.
- SourceMonoclonalMouseIgG1Clone#49106
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinanthumanCXCL9/MIG
Thr23-Thr125
Accession#Q07325 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelFluorescein
- IntracellularStainingbyFlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- Loetscher,M.etal.(1996)J.Exp.Med.184:963.
- Liao,F.etal.(1995)J.Exp.Med.182:1301.
- Vanguri,P.(1995)J.Neuroimmunol.56:35.
- EntrezGeneIDs:4283(Human);17329(Mouse);246759(Rat)
- AlternateNames:chemokine(C-X-Cmotif)ligand9;CMK;crg-10;C-X-Cmotifchemokine9;CXCL9;Gamma-interferon-inducedmonokine;Humig;MIG;MIGSmall-inducIBLecytokineB9;monokineinducedbygammainterferon;SCYB9Monokineinducedbyinterferon-gamma
Background:
CXCL9,amemberofthealpha subfamilyofchemokinesthatlacktheELRdomain,wasinitiallyidentifiedasalymphokine-activatedgeneinmousemacrophages.HumanCXCL9wassubsequentlyclonedusingmouseMIGCDNAasaprobe.TheCXCL9geneisinducedinmacrophagesandinprimaryglialcellsofthecentralnervoussystemspecificallyinresponsetoIFN-gamma. CXCL9 hasbeenshowntobeachemoattractantforactivatedT-lymphocytesandTILbutnotforneutrophilsormonocytes.ThehumanCXCL9cDNAencodesa125 aminoacid(aa)residueprecursorproteinwitha22aaresiduesignalpeptidethatiscleavedtoyielda103aaresiduematureprotein.CXCL9hasanextendedcarboxy-terminuscontaininggreaterthan50%basicaaresiduesandislargerthanmostotherchemokines.Thecarboxy-terminalresiduesofCXCL9arepronetoproteolyticcleavageresultinginsizeheterogeneityofnaturalandrecombinantCXCL9.CXCL9withlargecarboxy-terminaldeletionshavebeenshowntohavediminishedactivityinthecalciumfluxassay.Achemokinereceptor(CXCR3)specificforCXCL9andIP-10has beenclonedandshowntobehighlyexpressedinIL-2-activatedT-lymphocytes.TheE.coli-expressedCXCL9preparationsproducedatR&DSystemshavebeenshowntocontaingreaterthan80%fulllengthCXCL9.
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我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因


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