- SpeciesReactivityMouse
- SpecificityDetectsmouseIL-2inflowcytometry.
- SourceMonoclonalRatIgG2BClone#JES6-5H4
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinantMouseIL-2
- FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelFluorescein
- IntracellularStainingbyFlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- LongName:Interleukin2
- EntrezGeneIDs:3558(Human);16183(Mouse);116562(Rat);396868(Porcine);280822(Bovine);403989(Canine);100034204(Equine);751114(Feline);100302458(Rabbit)
- AlternateNames:aldesleukin;IL2;IL-2;IL-2lymphokine;interleukin2;interleukin-2;involvedinregulationofT-cellclonalexpansion;Tcellgrowthfactor;T-cellgrowthfactor;TCGF
Background:
Interleukin-2(IL-2)isanO-glycosylatedfouralpha-helixbundlecytokinethathaspotentstimulatoryactivityforantigen-activatedT cells.ItisexpressedbyCD4+andCD8+Tcells,gammaδTcells,Bcells,dendriticcells,andeosinophils.MaturemouseIL-2shares56% and73% aasequenceidentitywithhumanandratIL-2,respectively.Itshowsstrain-specificheterogeneityinanN-terminalregionthatcontainsapoly-glutaminestretch.MouseandhumanIL-2exhibitcross-speciesactivity.ThereceptorforIL-2consistsofthreesubunitsthatarepresentonthecellsurfaceinvaryingpreformedcomplexes.The55kDaIL-2RalphaisspecificforIL-2andbindswithlowaffinity.The75kDaIL-2Rbeta,whichisalsoacomponentoftheIL-15receptor,bindsIL-2withintermediateaffinity.The64 kDacommongammachaingammac/IL-2Rgamma,whichissharedwiththereceptorsforIL-4,-7,-9,-15,and-21,doesnotindependentlyinteractwithIL-2.Uponligandbinding,signaltransductionisperformedbybothIL-2Rbetaandgammac.IL-2isbestknownforitsautocrineandparacrineactivityonT cells.ItdrivesrestingTcellstoproliferateandinducesIL-2andIL-2Ralphasynthesis.ItcontributestoTcellhomeostasisbypromotingtheFas-induceddeathofnaïveCD4+TcellsbutnotactivatedCD4+memorylymphocytes.IL-2playsacentralroleintheexpansionandmaintenanceofregulatoryTcells,althoughitinhibitsthedevelopmentofTh17polarizedcells.Thus,IL-2maybeakeycytokineinthenaturalsuppressionofautoimmunity.
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1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转

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