Polypropylene solutionbasin/reagent reservoir has a standard 50 mL basin on one side and 12individual 5 mL basins on the other side. Wells are angled both downward andoutward, guiding multichannel pipettor tips toward a centered position, whichhelps to avoid contamination and waste. Imprinted indices identify samples;loose-fitting lids prevent evaporation and contamination. Non-sterile. Pack of25 reservoirs and 25 lids.
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因

