SavebypurchasingbulkquantitiesoftheMasterPure™buffersandreagents
Save:Reducecostsbypurchasingtheseimportantreagentsinbulkquantities- Scale-up:Usetheselargevolumesofreagentstoperformlarge-scaleMasterPure™purifications
ProteinaseK:UsefulformultipleapplicationsoutsideoftheMasterPure™Kitsincluding(i)isolationofplasmidsandhighmolecularweightgDNA,purificationofintactRNA,andinactivationofRNases,DNasesandotherenzymesinmolecularBIOLOGyreactions(seebelow)
RedCellLysisSolution:Selectivelylyseredbloodcellswhileleavingwhitebloodcellsintact
TissueandCellLysisSolution:UseincombinationwithProteinaseKtolysecellsandtissuesinvarioussamplessuchassaliva,semen,mammaliancells,andgramnegativebacteria
MPCProteinPrecipitationSolution:Asimplesolutionandmethodfortheremovalofproteinsfromcelllysatespriortonucleicacidpurification
TEBuffer:Standardbufferforstoringnucleicacids(10 mMTris-HCl[pH 7.5],1 mMEDTA)
DNaseI,RNase-Free:RemoveunwantedDNAfromRNAsampleswithoutdegrADIngpreciousRNA
RNaseA:DigestunwantedRNAinvarioussamples
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甲液:0.05mol/L Na2HPO4溶液 :称取磷酸氢二钠9.465g 7.099g,加蒸 馏水至1000ml ;
乙液:0.05mol/L KH2P04溶液: 称取磷酸二氢钾, 9.07g 6.803g,加蒸馏水至1000m1.
将甲乙液分装在棕色瓶内,于4℃冰箱中保存,用时甲、乙两液各按不同比例混合,即可得所需pH的缓冲液,见下表:
pH 甲液ml 乙液mI
5.29 2.5 97.5
5.59 5.0 95.0
5.91 10.0 90.0
6.24 20.0 80.0
6.47 30.0 70.0
6.64 40.0 60.0
6.81 50.0 50.0
6.98 60.0 40.0
7.17 70.0 30.0
7.38 80.0 20.0
7.73 90.0 10.0
8.04 95.0 5.0
你要配制pH=6.8的PBS,可用甲、乙液各50ml混合即可.
(2)酸和盐浓度等比例也增减时,溶液的pH值不便。
(3)酸和盐浓度相等时,缓冲液的缓冲效率为最高,比例相差越大,缓冲效率越低,一般地说缓冲液有效缓冲范围为PK±1pH

