
MultiBOOSTER
Assay Diluent
Cat. No. 5340
Every single step of an immunoassay, is individually optimized to secure maximum assay performance, and a good assay buffer plays a key role in the different layers of an immunoassay. MultiBOOSTER is developed to secure maximum sensitivity and minimal background. The buffer can be used as a diluent for:
- Sample matrices
- Dissolving and diluting lyophilized standards and controls
- Free poly- and monoclonal antibodies & Antigens
- HRP-conjugated antibodies & Antigens
- AP-conjugated antibodies & Antigens
- Fluorescence-conjugated antibodies & Antigens
The buffer amplifies the specific bindings between the layers. A significant boosting effect is achieved using the MultiBOOSTER for the dilution of the Secondary Antibody. Using the MultiBOOSTER as a multilayer enhancer offers a detection limit improvement up to 4 fold.
The product is available in bulk quantities.
Specifications
- Available sizes:500 mL, 1 L, bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C
- Stability:3 years
Downloads
Download your LOT specific Certificate of Analysis (CoA)

ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
ebiomall.com






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(2)酸和盐浓度等比例也增减时,溶液的pH值不便。
(3)酸和盐浓度相等时,缓冲液的缓冲效率为最高,比例相差越大,缓冲效率越低,一般地说缓冲液有效缓冲范围为PK±1pH
酶提取技术,属地球化学勘查学科。其是由克拉克(J.R.Clark)等人于20世纪80年代末和90年代初研制出的一种利用葡萄糖氧化酶提取矿物颗粒表面的非晶质锰的氧化膜寻找隐伏矿的方法。1995年以后已广泛应用。
2.尿素:有膜蛋白的损失
3.RIPA:用于磷酸化蛋白的温和裂解,不破坏磷酸基团
甲液:0.05mol/L Na2HPO4溶液 :称取磷酸氢二钠9.465g 7.099g,加蒸 馏水至1000ml ;
乙液:0.05mol/L KH2P04溶液: 称取磷酸二氢钾, 9.07g 6.803g,加蒸馏水至1000m1.
将甲乙液分装在棕色瓶内,于4℃冰箱中保存,用时甲、乙两液各按不同比例混合,即可得所需pH的缓冲液,见下表:
pH 甲液ml 乙液mI
5.29 2.5 97.5
5.59 5.0 95.0
5.91 10.0 90.0
6.24 20.0 80.0
6.47 30.0 70.0
6.64 40.0 60.0
6.81 50.0 50.0
6.98 60.0 40.0
7.17 70.0 30.0
7.38 80.0 20.0
7.73 90.0 10.0
8.04 95.0 5.0
你要配制pH=6.8的PBS,可用甲、乙液各50ml混合即可.
百度教育团队【海纳百川团】为您解答。
感谢您的采纳 O(∩_∩)O 。如有疑问,欢迎追问。

