- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype: Mouse IgG1k
Specifcity:T-2 mycotoxin
Form:The low endotoxin, azide-free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Storage:Store at 2 – 8 °C. Aliquot and store at or below -20°C.
Literature/Support
PDF Insert
How To Use
Applications:
- Used for Flow cytometry
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA)
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA)
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs
Related Products
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根据组成不同,可分为两种,弱酸及其对应的强碱弱酸盐,弱碱及其对应的强酸弱碱盐。
因为HF可以和NaOH反应生成NaF和水,当NaOH反应完之后,NaF就可以与HF组成缓冲溶液,所以说可以直接使用NaOH和HF来配制缓冲溶液。
这也是一般配制缓冲溶液的方法,也就是用强碱和弱酸(或者强酸和弱碱)来配制缓冲溶液。
(2)酸和盐浓度等比例也增减时,溶液的pH值不便。
(3)酸和盐浓度相等时,缓冲液的缓冲效率为最高,比例相差越大,缓冲效率越低,一般地说缓冲液有效缓冲范围为PK±1pH
浙江大学奚振宇、徐又一、朱利平等2009年在journal of membrane science(中文翻译为《膜科学杂志》)发表的一篇论文,其中探讨了缓冲溶液的pH值对聚乙烯-多巴胺复合膜亲水性的影响,发现缓冲溶液的pH值为8.5时制备的复合膜亲水性最强。
通过大量的实验,研究人员发现,pH值为8.5时制备的多巴胺复合膜性能最优。这个是通过大量的实验总结得到的结论。
2.尿素:有膜蛋白的损失
3.RIPA:用于磷酸化蛋白的温和裂解,不破坏磷酸基团