DescriptionViPrimePLUS Taq qPCR Green Master Mix I is next generation master mix designed for fast and easy realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains all the reagents, including Taq DNA Polymerases, SYBR® Green dye, highest quality dNTPs, and buffer components at optimal concentrations. Taq DNA Polymerases in the master mix provide excellent results in reaction efficiency, correlation coefficient and slope. SYBR® Green dye emits fluorescence when bound to double-stranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.ViPrimePLUS Taq qPCR Green Master Mix I can be used to amplify any DNA template including genomic, cDNA and viral sequences. The formulation of qPCR green master mix can detect low copy number targets very specifically with high efficiency. The qPCR green master mix provides convenient and robust set up for quantitative real-time analysis of DNA samples.ViPrimePLUS Taq qPCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS Taq qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of sample material suited for qPCR amplification can be used.
Features
- Ready-to-use real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Good buffer system for excellent amplification efficiency
- Includes SYBR® Green dye for intercalator-based qPCR
- Increased limit of detection
- Compatible with most of the real-time PCR platforms
Component1.6ml aliquots of master mix
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix I has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM12ViPrimePLUS Taq qPCR Green Master Mix I (SYBR® Green Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM12-LRViPrimePLUS Taq qPCR Green Master Mix I with Low ROX (SYBR® Green Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM12-RViPrimePLUS Taq qPCR Green Master Mix I with ROX (SYBR® Green Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM12 | ViPrimePLUS Taq qPCR Green MasterMix I (SYBR® Green Dye) | 150 reactions |
| QLMM12-LR | ViPrimePLUS Taq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) | 150 reactions |
| QLMM12-R | ViPrimePLUS Taq qPCR Green MasterMix I with ROX (SYBR® Green Dye) | 150 reactions |
DownloadManual
ViPrimePLUS Taq qPCR Green MasterMix I (SYBR® Green Dye) ViPrimePLUS Taq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) ViPrimePLUS Taq qPCR Green MasterMix I with ROX (SYBR® Green Dye)
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1、根据所需求的酸碱性选择合适的缓冲对，若是配制酸性缓冲液就选择弱酸与弱酸盐缓冲对；若是配制碱性缓冲液就选择弱碱与弱碱盐缓冲对。
2、根据所需要控制的PH范围以及弱酸和弱碱的解离常数（pKa/pKb）选择具体的共轭酸碱对，公式为PH＝pKa±1＝（14-pKb）±1。
3、根据公式计算缓冲溶液的组分比，酸性缓冲液PH＝pKa－lgc酸/c盐，碱性缓冲液PH＝14-pKb+lgc碱/c盐。
4、根据共轭酸碱对以及其组分比配制缓冲液，方法同普通溶液。
举例：试配制一种缓冲液，体积为1L，PH能维持在10.25左右。
a、依题意选择弱碱与弱碱盐的共轭对；
b、由PH＝（14-pKb）±1，算出pKb在3.75与4.75之间，查弱碱的解离常数表可知氨水（pKb=4.75）符合要求，故可选择NH3-NH4Cl体系；
c、由PH＝14-pKb+lgc碱/c盐，算出c(氨水)/c(氯化铵)＝10；
d、设氨水的浓度为10mol/L，则氯化铵的浓度为1mol/L，所以在浓度为10mol/L体积为1L的氨水中加入1mol的氯化铵即可。
酶提取技术，属地球化学勘查学科。其是由克拉克（J.R.Clark）等人于20世纪80年代末和90年代初研制出的一种利用葡萄糖氧化酶提取矿物颗粒表面的非晶质锰的氧化膜寻找隐伏矿的方法。1995年以后已广泛应用。
根据组成不同，可分为两种，弱酸及其对应的强碱弱酸盐，弱碱及其对应的强酸弱碱盐。
因为HF可以和NaOH反应生成NaF和水，当NaOH反应完之后，NaF就可以与HF组成缓冲溶液，所以说可以直接使用NaOH和HF来配制缓冲溶液。
这也是一般配制缓冲溶液的方法，也就是用强碱和弱酸（或者强酸和弱碱）来配制缓冲溶液。
