Product Specifications:
Item#1005-G: gag p17 HIV-1 IIIB Conjugated to Colloidal Gold
Concentration: See vial
Volume/vial: 1ml Gold Conjugate
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3
Storage: 4°C
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: Dot Blot Assay-Anti-p17 Antibody Binding, Immunohistochemistry.
Description: rp17 HIV-1 IIIB (E.coli) conjugated to Colloidal Gold (25nm mean particle size).
Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.
Specificity: Gold conjugated p17 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays. Reaction time: 5 - 10 minutes at room temoperature.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.
2. Immunohistochemical studies may be performed in 1 : 50 dilution range.
Related Products:
Item#: 1005-P Peroxidase-Conjugated p17 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 1005-F Flourescene-Conjugated p17 HIV-1 IIIB
Application: FACS analysis, Histocytology.
Item#: 1005-M Magnetically-Coupled p17 HIV-1 IIIB
Application: Human diagnostics, Human B and T-cell panning.
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磷脂C、H、O、N、P固醇C、H、ODNA 和RNA一样 CHONP
第一类激素作用在靶细胞表面,并不进入细胞内部,而是与细胞膜表面特异的受体结合.这种结合使腺苷酸环化酶激活产生cAMP(一种第二信使),cAMP再去激活细胞内的一些特定系列的酶,从而引起各种生理效应.这是由E.W.Sutherland于1965年提出来的第二信使假说
第二类激素由于是脂溶性的小分子.能直接进入靶细胞,与靶细胞的细胞质中的受体分子结合成"激素-受体复合物",在一定条件下穿过核膜进入核内,与染色质上的一种酸性蛋白质相互作用,促进DNA样板转录相应的mRNA.
mRNA扩散出核膜进入细胞质,导致某种蛋白质(酶)的合成,从而引起这种激素的生理效应.

