
GradeMolecular Biology Grade
DescriptionDNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.
Specification
Appearance | White to off-white crystalline powder; a partially purified, lyophilized powder |
Source | Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation |
Unit Definition | One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit. |
StorageStore at -20°C
Assay | Resuls |
Activity | >500Kunitz U/mg |
Loss on Drying | ≤5.0% |
Residue on Ignition | ≤1.0% |
Ordering Information
Catalog No | Description | Pack Size |
PC0704-25mg | Deoxyribonuclease I (DNase I) | 25mg |
PC0704-50mg | Deoxyribonuclease I (DNase I) | 50mg |
PC0704-100mg | Deoxyribonuclease I (DNase I) | 100mg |
PC0704-1g | Deoxyribonuclease I (DNase I) | 1g |
DownloadMSDS
Deoxyribonuclease I (DNase I)
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这个“类”是什么意思?
多肽和蛋白质类激素(不可口服):促甲状腺激素释放激素,促甲状腺激素,促性腺激素、生长激素,催乳素,胰岛素,胰高血糖素,
氨基酸类衍生物(可口服):甲状腺激素、肾上腺素
脂质类激素:(可口服)性激素和孕激素
蛋白质类激素不能口服,因会被消化液中的蛋白酶分解(——这是原因)
这是我们老师说的哦!!希望对你有帮助
作者、斯坦福大学医学中心的梅斯勒(AnnaH.Messner)和同事解释,有多个研究曾检查了在扁桃体切除手术前使用类固醇类药的情况,但得到的结果不一。据他们在8月《耳鼻喉、头、颈外科文献》(ArchOtolaryngolHeadNeckSurg2004;130:917-921)上发表的论文说,他们对这种病人进行了一项最大的前瞻性、随机、双盲研究。
共219名9个月至12岁的参试者被随机分为或用地塞米松1mg/kg,最大剂量至50mg(n=106),或用安慰剂(n=113)。在药物组中,62人进行的扁桃体切除术采用的是电烧烙法,44人采用的是锐性分离。安慰剂两种方法分别为56人和57人。术后第一天,类固醇组医生评定的Wong-Baker可视模拟量表上的疼痛分平均为4.4,安慰剂组5.3(p<0.001);前组家长评估的疼痛分(p=0.002)和病儿自己评估的疼痛分((p=0.002))也明显更低。类固醇组有1.2次呕吐发作,安慰剂组2.1次(p=0.02),前组也更快地恢复了正常进食(=0.004)。另外,作者还发现,进行锐性分离的病人使用类固醇受益最大,医生和家长评估的疼痛分显著低于电烧烙组。
“我们的研究结果支持在进行扁桃体手术时以1mg/kg的剂量IV地塞米松”,研究者总结。
http://care.diabetesjournals.org/cgi/content/abstract/31/8/1479
OBJECTIVE—Hyperglycemiaisariskfactorformicrovascularcomplicationsandmayincreasetheriskofcardiovasculardiseaseinpatientswithtype2diabetes.ThisstudytestedtheLDLcholesterol–loweringagentcolesevelamHCl(colesevelam)asapotentialnoveltreatmentforimprovingglycemiccontrolinpatientswithtype2diabetesonsulfonylurea-basedtherapy.
RESEARCHDESIGNANDMETHODS—A26-week,randomized,double-blind,placebo-controlled,parallel-group,multicenterstudywascarriedoutbetweenAugust2004andAugust2006toevaluatetheefficacyandsafetyofcolesevelamforreducingA1Cinadultswithtype2diabeteswhoseglycemiccontrolwasinadequate(A1C7.5–9.5%)withexistingsulfonylureamonotherapyorsulfonylureaincombinationwithadditionaloralanti-diabetesagents.Intotal,461patientswererandomized(230givencolesevelam3.75g/dayand231givenplacebo).Theprimaryefficacymeasurementwasmeanplacebo-correctedchangeinA1Cfrombaselinetoweek26intheintent-to-treatpopulation(lastobservationcarriedforward).
RESULTS—Theleastsquares(LS)meanchangeinA1Cfrombaselinetoweek26was–0.32%inthecolesevelamgroupand+0.23%intheplacebogroup,resultinginatreatmentdifferenceof–0.54%(P<0.001).TheLSmeanpercentchangeinLDLcholesterolfrombaselinetoweek26was–16.1%inthecolesevelamgroupand+0.6%intheplacebogroup,resultinginatreatmentdifferenceof–16.7%(P<0.001).FurThermore,significantreductionsinfastingplasmaglucose,fructosamine,totalcholesterol,non–HDLcholesterol,andapolipoproteinBweredemonstratedinthecolesevelamrelativetoplacebogroupatweek26.
CONCLUSIONS—ColesevelamimprovedglycemiccontrolandreducedLDLcholesterollevelsinpatientswithtype2diabetesreceivingsulfonylurea-basedtherapy.

