
- Replacement tips for KWIK-TIP Ion Selective Electrodes
Replacement tips
Set of 3 calcium replacement tips for ion selective electrodes. Change the tip on your holder in less than a minute.
Notes
The tips should be stored dry under refrigeration prior to use. Once filled, the membrane tip can last up to 30 days and be used for that length of time. The tip is fragile and the “membrane” gets softer when wetted. After use, the tips can be placed into storage. Before storage, wash the exterior and carefully blot it dry. Leave the filling solution inside the electrode and seal it with parafilm. Then, place it in a dry container and store it in the refrigerator.
The KWIK Ag/AgCl pellet should be washed in distilled water, dried and stored in a dry, dark location. If desired, you may change to a new ionic solution, however, it takes time for the old ionic solution to exchange out of the KWIK pellet and be replaced with the new ionic concentration.This should be done in a small beaker and the contaminated solution should be discarded. The new tip and electrode should be filled with fresh solution.
Kwik-Tip Instruction Manual
Electrode | Color Code | Filling Solution | Nim. Slope/Decade | Concentration Range | Selectivity Coeffiecients (-log) | |
TIPCA | Calcium | Green | 0.1M CaCl2 | 28mV | 0.1 - 10-6.75M | Na+ 5.5, K+ 5.4, Mg++ 4.9 |
TIPH | Hydrogen | Orange | 1M Citric acid, 0.01M NaCl, pH 5.6 | 54mV | pH 5.0-12 | Na+ 10.4, K+ 9.8, Ca++ 11.1 |
TIPK | Potassium | Yellow | 0.1M KCl | 54mV | 0.1 - 10-4.5M | Na+ 4.0, Ca++ 3.9, Mg++3.0 |
TIPTPP | TPP+ | Purple | 10mM TPP+ | 54mV | 0.001 - 10-4M | K+ 6.0 |
- Dri-Ref Reference Electrode, 4.7 mmDRIREF-5For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, 4.7 mm (short)DRIREF-5SHFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode with Luer TipDRIREF-LFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Flexible Dri-Ref Reference ElectrodeFLEXREFFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Electrode Holder for KWIK-TipsKWIK-2For pricing, Customers outside of the US and Canada, please contact your distributor.
- Micro Dri-Ref Reference ElectrodeDRIREF-450For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, 2 mmDRIREF-2For pricing, Customers outside of the US and Canada, please contact your distributor.
- Dri-Ref Reference Electrode, ShortDRIREF-2SHFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Hydrogen Electrode Tip, ReplacementTIPHFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Super Dri-Ref Reference ElectrodeSDR2For pricing, Customers outside of the US and Canada, please contact your distributor.
- TPP Electrode Tips, ReplacementTIPTPPFor pricing, Customers outside of the US and Canada, please contact your distributor.
- Potassium Electrode Tips, ReplacementTIPKFor pricing, Customers outside of the US and Canada, please contact your distributor.
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是欢快活泼的光辉色彩,是暖色系中最温暖的色,它使人联想到金色的秋天,丰硕的果实;
是一种富足、快乐而幸福的颜色。让人感觉成一种稳重、含蓄又明快的温暖;
橙色也会带来一种甜甜的感觉。
颜料是用着色的物质通常与被着色的物质混合在一起主要以无机化合物为主;染料是一种用来直接或经媒染剂作用而能附着在各种纤维和其他材料上的有色物质有的可以跟被染物质化合,多以有机化合物为主。 颜料不能上色,而染料能上色. 颜料和染料的区别 颜料是一种微细粉末状的有色物质,一般不溶于水、油和溶剂,但能均匀的分散在其中。颜料是色漆的次要成膜物质,在木材装饰过程中调制底漆、腻子以及木才着色,也经常使用颜料.
1.小鼠Lewis肺癌细胞DNA含量测定方法
(1).从C57BL/6小鼠上切除肿块,在培养皿内用PBS冲洗.
(2).去除结缔组织及脂肪,剪碎肿块.
(3).小碎片移入1.20×38mm注射针,加压使其通过,于4℃条件下重悬细胞于HBSS中.
(4).将200~300μL细胞悬液(5×105细胞/mL)中加入3mL PI(50μg/mL),染色3LL细胞,于4℃存放20~30分钟.
(5).测定580~750nm之间的发射荧光,以去除末结合PI产生的激发光与发射光谱线之间的重叠部分.
这些染料都非常成熟,光毒和淬灭都很低,当然要考虑到你采集图像时的显微镜参数。
比如calcein,常用的示踪,绿光(虽然这些颜色只是根据光谱加上去的伪彩),但要考虑你的实验过程中结合细胞结构,是否会伴随calcein的泄漏,就是荧光降低。
dri,还可以看膜啊
cfda也不错
bcef虽是ph指示,但你试验时不仅可观察细胞,还可看细胞ph变化,也行。
当然你或许还要结合其他方法,如细胞免疫化学等手段去双染或多染,都需要综合考虑染料之间特性。单独染一个染料,有点浪费,不如多染,数据和图像也好看些。现在流行细胞成像。
然后是碱性荧光染料,造纸厂用来增加纸的亮度。
直接和分散的荧光黄,印染厂用来增加棉质和化纤面料的艳度

