
- SpeciesReactivityHuman,Mouse
- SpecificityDetectshumanATG7indirectELISAsandWesternblots.IndirectELISAsandWesternblots,nocross-reactivitywithrecombinanthumanATG3,4A,4B,5,10,12,or16L1isobserved.
- SourceMonoclonalMouseIgG1Clone#683906
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinanthumanATG7
Asn387-Gln570
Accession#O95352 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelAlexaFluor594
- IntracellularStainingbyFlowCytometry5µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- LongName:ATG7AutophagyRelated7Homolog
- EntrezGeneIDs:74244(Mouse);312647(Rat);10533(Yeast);10533(Yeast)
- AlternateNames:APG7autophagy7-like(S.cerevisiae);APG7;APG7L;APG7-LIKE;ATG12-activatingenzymeE1ATG7;ATG7autophagyrelated7homolog(S.cerevisiae);ATG7;Autophagy-relatedprotein7;DKFZp434N0735;GSA7;hAGP7;ubiquitinactivatingenzymeE1-likeprotein;Ubiquitin-activatingenzymeE1-likeprotein;ubiquitin-likemodifier-activatingenzymeATG7
Background:
ATG7,alsoknownasAPG7,isa78kDacytosolicubiquitin-E1-likeenzymethatplaysakeyroleintheautophagicpathwayofintracellularbulkdegradation.ItisrequiredfortheconjugationofATG5toATG12,thelipidationofATG8,andsubsequentautophagosomeformation.ATG7isrequiredformitochondrialremovalduringerythropoiesisandforthemaintenanceofaxonalhomeostasis.AlternatesplicingofhumanATG7generatesanisoformthatlacks31aaattheC-terminus,aregionthatisrequiredforATG8lipidation.HumanATG7shares93%aasequenceidentitiywithmouseandratATG7.
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