- SpeciesReactivityHuman
- SpecificityDetectshumanIL-27indirectELISAs.
- SourceMonoclonalMouseIgG2AClone#307426
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanIL‑27
Arg21-Lys229ofEBI-3(Accession#Q14213.2)andPhe29-Pro243ofp28(Accession#AAM34498) - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelPhycoerythrin
- IntracellularStainingbyFlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- LongName:Interleukin27
- EntrezGeneIDs:246778(Human);246779(Mouse)
- AlternateNames:IL-27p28subunit;IL-27subunitalpha;IL27;IL-27;IL27A;IL-27-A;IL27-A;IL27p28;IL30;interleukin27;interleukin30;interleukin-27subunitalpha;MGC71873;p28IL-27A
Background:
IL-27isanon-covalentheterodimericligandmoleculethatbelongstotheIL-6/IL-12familyoflongtypeIcytokines.ItiscomposedofEBI3(EBV-inducedgene 3),a34kDaglycoproteinthatisrelatedtothep40subunitofIL-12andIL-23,andp28,the 28kDaglycoproteinthatisrelatedtothep35chainofIL-12.ThehumanEBI3geneencodesa229aminoacid(aa)precursorthatcontainsa20aasignalpeptideand209aamatureprotein.ThematureregioncontainstwopotentialN-linkedglycosylationsites,twofibronectintypeIIIdomains,andtwopairsofconservedcysteineresidueswithaWSXWS-likemotifthatplacesthemoleculeinthehematopoietinreceptorfamily.Althoughp40,theEBI3counterpartinIL-12,isknowntoformhomodimers,thereisnoevidencetodatethatEBI3alsohomodimerizes.HumanEBI3is61%aaidenticaltomouseEBI3.Thehumanp28geneencodesa243aaprecursorthatcontainsa28aasignalsequenceand215 aamatureregion.Thematureregionischaracterizedbythepresenceoffouralpha-helices,placingitintheIL-6familyofhelicalcytokines.Humanp28is74%aaidenticaltomousep28.IL-27isexpressedbymonocytes,endothelialcellsanddendriticcells.IL-27bindstoandsignalsthroughaheterodimericreceptorcomplexcomposedofWSX-1(TCCR)andgp130.EvidencesuggestsIL-27interactsonlywithWSX-1.IL-27hasbothanti-andproinflammatoryproperties.Asananti‑inflammatory,IL-27seemstoinduceageneralnegativefeedbackprogramthatlimitsTandNK-Tcellactivity.Attheonsetofinfection,IL-27inducesanIL‑12 receptoronnaïveCD4+Tcells,makingthemsusceptIBLetosubsequentIL-12activitythatgeneratesTh1cellsattheexpenseofTh2andTh17cells.Finally,IL-27upregulatesbothMHC-IIandchemokine(CXCL9;CXCL10)expressiononvascularendothelium,suggestingaroleforIL-27invascularinflammation.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
暂无品牌分类
