- Species ReactivityMouse
- SpecificityDetects mouse Wnt-5b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-5a, -8a, -8b, -9b, -10a, -10b, or recombinant human Wnt-7b is observed.
- SourceMonoclonal Rat IgG2A Clone # 325004
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant mouse Wnt‑5b
Ser55-Ser95 and Lys246-Glu315 connected by a Gly-Ser linker
Accession # NP_033551 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Immunocytochemistry8-25 µg/mLSee below
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Miller, J.R. (2002) Genome Biol. 3:3001.
- Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
- Veeman, M.T. et al. (2003) Developmental Cell 5:367.
- Gavin, B. J. et al. (1990) Genes Dev. 4:2319.
- Nusse, R. and H.E. Varmus (1992) Cell 69:1073.
- Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
- Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
- Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
- Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
- Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
- Yang, Y. et al. (2003) Development 130:1003.
- Long Name:Wingless-type MMTV Integration Site Family, Member 5b
- Entrez Gene IDs:81029 (Human)
- Alternate Names:MGC2648; protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein; Wnt5b; Wnt-5b
Background:
Wnt proteins are secreted glycoproteins with a conserved pattern of 23-24 cysteine residues that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1-3). Wnt-5b is a 49 kDa glycoprotein of the subgroup of Wnts that is implicated in the Wnt/Ca2+ pathway (3-6). It is not axis-inducing in Xenopus embryos and only weakly transforms C57MG mammary epithelial cells. The non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling (3). Mouse Wnt-5b is synthesized as a 359 amino acid (aa) precursor that contains a 17 aa signal sequence and a 342 aa mature region. It is ubiquitously expressed at low but increasing levels throughout embryonic development (4, 5, 7). In adult mice, Wnt-5b is expressed in heart, liver, brain, lung, testes, kidney, and pancreas (4, 8). Wnt-5b appears to promote adipogenesis. It is upregulated in early adipogenesis. Also, Wnt-5b overexpression in 3T3-L1 cells partially inhibits canonical Wnt suppression of adipogenesis (9, 10). Human Wnt-5b polymorphisms have been associated with Type II diabetes (9). Although Wnt-5a and Wnt-5b share 83% aa identity, they show differential expression and regulation of cyclin D1 and p130 during endochondral bone development. Together, they appear to coordinate chondrocyte proliferation and differentiation (11). Mature mouse Wnt-5b shows 94%, 98%, 90%, and 88% aa sequence identity with mature human, rat, chick, and Xenopus Wnt-5b, respectively.
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