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R&D Systems/Mouse CCL3/MIP-1 alpha AlexaFluor®488-conjugated Antibody/IC450G/100 Tests

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R&D Systems/Mouse CCL3/MIP-1 alpha AlexaFluor®488-conjugated Antibody/IC450G/100 Tests

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rndsystems

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IC450G-100Tests

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SpeciesReactivity
Mouse
Specificity
DetectsmouseCCL3/MIP‑1alphainWesternblots.InWesternblots, approximately25%cross-reactivitywithrecombinantmouseCCL4/MIP‑1beta isobserved andnocross-reactivitywithrecombinanthumanCCL1,2,3,5,7,8,11,13,14,15,16,17,18,21,22,23,24,25,28,recombinantmouseCCL1,2,6,7,9/10/MIP-1gamma,11,12,19,21,22,25,28,recombinantratCCL2orCCL20isobserved.NeutralizestheBIOLOGicalactivityofrecombinantmouseCCL3and willnotblockthebiologicalactivityofrecombinanthumanCCL3.
Source
MonoclonalRatIgG_2AClone#39624
Purification
ProteinAorGpurifiedfromhybridomaculturesupernatant
Immunogen
E.coli-derivedrecombinantmouseCCL3/MIP-1alpha
Ala24-Ala92
Accession#P10855
Formulation
SuppliedinasalinesolutioncontainingBSAandSodiumAzide.
Label
AlexaFluor488

IntracellularStainingbyFlowCytometry
5µL/10⁶cells
Seebelow

Shipping
Theproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
StABIlity&Storage
Protectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.

References:
1. Menten,P.etal.(2002)CytokineGrowthFactorRev.13:455.
EntrezGeneIDs:
6348(Human);20302(Mouse);25542(Rat);448787(Canine)
AlternateNames:
C-Cmotifchemokine3;MIP1-(a);AI323804;CCL3;chemokine(C-Cmotif)ligand3;G0S19-1;LD78a;LD78alpha;MIP1alpha;MIP-1alpha;Mip1a;MIP-1alpha;MIP1-alpha;Scya3

Background:

Themacrophageinflammatoryproteins1alphaand1beta,twocloselyrelatedbutdistinctproteins,wereoriginallyco-purifiedfrommediumconditionedbyaLPS-stimulatedmurinemacrophagecellline.MaturemouseCCL3/MIP-1alphasharesapproximately77%and70%aminoacididentitywithhumanCCL3/MIP-1alphaandmouseCCL4/MIP-1beta,respectively.MIP‑1proteinsareexpressedprimarilyinTcells,Bcells,andmonocytesafterantigenormitogenstimulation.TheMIP‑1proteinsaremembersofthebeta (C‑C)subfamilyofchemokines.

BothCCL3andCCL4aremonocytechemoattractantsinvitro.Additionally,theMIP‑1proteinshavebeenreportedtohavechemoattractantandadhesiveeffectsonlymphocytes,withCCL3andCCL4preferentiallyattractingCD8⁺andCD4⁺Tcells,respectively.CCL3hasalsobeenshowntoattractBcellsaswellaseosinophils.MIP‑1proteinshavebeenreportedtohavemultipleeffectsonhematopoieticprecursorcells,andCCL3hasbeenidentifiedasastemcellinhibitoryfactorthatcaninhibittheproliferationofhematopoieticstemcellsinvitroaswellasinvivo.Inthesameassays,CCL4wasreportedtobemuchlessactive.ThefunctionalreceptorforCCL3hasbeenidentifiedasCCR1andCCR5.

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