+,R&D Systems/Human PU.1/Spi-1 Fluorescein-conjugated Antibody/IC5870F/100 Tests,盐,R&D Systems,Bio-Techne,,100 Tests蚂蚁淘商城

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R&D Systems/Human PU.1/Spi-1 Fluorescein-conjugated Antibody/IC5870F/100 Tests

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R&D Systems/Human PU.1/Spi-1 Fluorescein-conjugated Antibody/IC5870F/100 Tests

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rndsystems

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IC5870F-100Tests

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Species Reactivity
Human
Specificity
Detects human PU.1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human SPI-B is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PU.1
Met1-Lys169
Accession # NP_001074016
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Intracellular Staining by Flow Cytometry
10 µL/10⁶ cells
See below

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.

Long Name:
Hematopoietic transcription factor PU.1
Entrez Gene IDs:
6688 (Human); 20375 (Mouse); 366126 (Rat)
Alternate Names:
hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SFPI131 kDa transforming protein; SPI-1 proto-oncogene; SPI1; Spi-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein; transcription factor PU.1

Background:

PU.1 (Purine-rich nucleic acid binding protein 1; also 31 kDa transforming protein and SPI-1) is a member of the PU subfamily, ETS family of transcription factors. Although its predicted MW is 31 kDa, it appears to run anomalously high in SDS-PAGE at 40‑45 kDa. PU.1 is a monomeric hematopoietic protein that regulates the differentiation of early myeloid and lymphoid progenitors. High PU.1 levels favor granulocyte and macrophage production, while low levels generate magakaryocytes, erythrocytes, plus T and B cells. Human PU.1 is 270 amino acids (aa) in length. It contains an N-terminal acidic/polyGln transactivation region (aa 34‑99), a non‑destabilizing PEST sequence (aa 117‑165) and a C-terminal Ets DNA-binding domain (aa 170‑253). PU.1 is phosphorylated on Ser146, allowing it to bind to Pip. Over aa 1‑169, human PU.1 shares 88% aa identity with mouse PU.1.

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