- Species ReactivityHuman
- SpecificityDetects human PAWR in direct ELISAs and Western blots.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human PAWR
Arg2-Ala121
Accession # CAD88640 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Immunocytochemistry5-15 µg/mLSee below
- ReconstitutionSterile PBS to a final concentration of 0.2 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:PRKC, Apoptosis, WT1, Regulator
- Entrez Gene IDs:5074 (Human); 114774 (Mouse); 64513 (Rat)
- Alternate Names:PAR4; PAWR; WT1 Interacting Protein
Background:
PAWR (PRKC Apoptosis WT1 Regulator protein; also PAR-4) is an intracellular, 38-42 kDa pro-apoptotic protein. It is widely expressed, and serves multiple functions. WT1 protein is both a transcriptional activator and repressor. When complexed to PAWR, WT1 activation function is repressed, while its repressor activity is enhanced. Thus, PAWR generates transcriptional repression. PAWR also binds to the atypicallambda PKC andzeta PKC isotypes. Such binding inhibits PKC avtivity, blocks cell division and MAPK activation, and promotes Fas-mediated cell apoptosis. Finally, in neurons, PAWR binds to BACE1, promoting the cleavage of APP. Human PAWR is 340 amino acids (aa) in length. It contains an Ala-rich region (aa 49-120), an NLS (aa 145-161), one coiled-coil region (aa 186-206), and a Leu-zipper domain (aa 300-340). There are at least five utilized Ser/Thr phosphorylation sites. PAWR forms noncovalent homodimers and is reported to homooligomerize. There is one potential splice form that shows a three aa substitution for aa 173-340, and a P-P-A-R substitution for A102P103. Full-length PAWR shares 78% aa identity with mouse PAWR.
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