
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Ub-AQUA analysis:
K11: 98.87%
K63: 0.82%
K33: 0.11%
K48: 0.11%
K6 : 0.08%
All other linkages ≤ 0.01%
- Activity
Ubiquitin chains vary in length, linkage, and function. K11-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40>
- SourceE. coli-derived
- Accession #
- Predicted Molecular Mass34 kDa
- Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
- Ordureau, A., et al. (2014) Mol. Cell 56(3): 360–375
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
- Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
- Entrez Gene IDs:7314 (Human)
- Alternate Names:TetraUbiquitin; Tetra-Ubiquitin; Ub4
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