4% B Agarose Bead Standard is a non-crosslinked agarose resin with a bead size of 50-150 µm. It is a resin commonly used for gel filtration as well coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.
REFERENCES:
- Franziska Hagemann, Patrick Adametz, Matthias Wessling, Volkmar Thom, "Modeling hindered diffusion of antibodies in agarose beads considering pore size reduction due to
adsorption." Journal of Chromatography A (2020) - Kato, Shinji et al. “The Membrane Phospholipid Cardiolipin Plays a Pivotal Role in Bile Acid Adaptation by Lactobacillus Gasseri JCM1131 T.” Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1864.3 (2019): 403–412. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. Web.
| Product Name | 4% B Agarose Bead Standard (50-150µm) |
| Cat. No. | A-1040S-X |
| Bead Geometry & Size | Spherical ~50-150 µm |
| Bead Mean Diameter d50v | ~90 µm |
| Crosslinked | No |
| Agarose % | 4% |
| Linear Recommended Flow Rate | <11.5> |
| Fractionation (mw) Globular Proteins Da | 7x10^4 - 2x10^7 |
| Exclusion Limit Da | > 2x10^7 |
| pH Stability Working Range | 4-9 |
| ph stability cleaning in place (cip) | 4-9 |
| Chemical Stability | Stable in moderate acid and basic solutions |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-30˚C |
ABTBeads提供一系列优质规则的活化琼脂糖微粒，用于亲和层析、SEC（体积排斥色谱）、固定化和多种纯化。产品主要涉及HIS-TAG融合蛋白的纯化，抗体纯化，GST融合蛋白纯化，酶和抗体的固定化，体积排斥色谱等方面的琼脂糖微粒。及一些分子生物学级电游试剂和用于蛋白纯化用的柱子。
产品列表
AFFINITYCHROMATOGRAPHY
HIS-TAGPURIFICATION HIS-TAG融合蛋白的纯化
•Chelatingagarosebeads
•ChelatingagarosebeadsNTA
•ChelatingRapidRun™agarosebeads
NEW:5mlCARTRIDGES!
ANTIBODYPURIFICATION 抗体纯化
•ProteinAagarosebeads
•ProteinARapidRun™agarosebeads
GSTPURIFICATION GST融合蛋白纯化
•Glutathioneagarosebeads
ENZYME&ANTIBODYIMMOBILIZATION 酶和抗体的固定化
AMINOGROUPS
•Glyoxalbeads
•GlyoxalRapidRun™agarosebeads
ACIDICGROUPS
•Aminoethylbeads
•AminoethylRapidRun™agarosebeads
SIZEEXCLUSIONCHROMATOGRAPHY 体积排斥色谱
LOWPRESSURE
•PlainandCrosslinkedAgarosebeads
HIGHPRESSURE
•RapidRun™Agarosebeads
ELECTROPHORESISREAGENTS 电游试剂
AGAROSEPOWDER
•AgaroseLEMolecularBIOLOGyGrade
•AgaroseHRMolecularBiologyGrade
•AgaroseGAElectrophoresisGrade
•AgaroseLMMolecularBiologyGrade
ACCESSORIES 纯化用柱子等辅助产品
•Emptycolumns
•Emptyspincolumns
•Minicolumns
•Emptycartridges
ABTBeads提供一系列优质规则的活化琼脂糖微粒，用于亲和层析、SEC（体积排斥色谱）、固定化和多种纯化。产品主要涉及HIS-TAG融合蛋白的纯化，抗体纯化，GST融合蛋白纯化，酶和抗体的固定化，体积排斥色谱等方面的琼脂糖微粒。及一些分子生物学级电游试剂和用于蛋白纯化用的柱子。
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最近在做小鼠基因型鉴定，之前做出来的效果很好，没有杂带，只有明显的目的条带，但是最近几次老是出现很多杂带，但是还是看得清目的条带，不影响判断。想请问一下有经验的朋友，这可能是什么原因引起的呢？该怎么避免？谢谢！
TBE作为电泳缓冲液，其中的离子可以导电。并且TE作为DNA的储存液，可以保证DNA免受DNA酶的降解，其中的离子也可以稳定DNA的存在。
biowest agarose 指的是西班牙琼脂糖。
agarose指的是琼脂糖。
手提的斑马鱼RNA再反转录得到CDNA作为模板进行primestar,p的是斑马鱼的全长，但是全长p出来总是带有拖带的情况，有一个情况较好的图是这样的（mark左边的那一条）
我就用这个pcr的产物作为模板又做了一次，结果如下
求大神赐教！！！
请问各位大神，配制琼脂糖胶的电泳浓度是怎么设定，比如百分之1或百分之1.5的浓度是怎么设置，是1.5g的琼脂糖粉加100g的TAE麽？
