
Source | M.W. | ~45 kDa | CAS No. | ||
---|---|---|---|---|---|
Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum. | ||||
Stability | The lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at least 2weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze/thaw. | ||||
Purity | Greater than 85% as determined by SDS-PAGE and HPLC analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay. | ||||
Country of Origin | USA |
WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of human WNT-5a is a monomeric protein containing 357 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa on SDS-PAGE undernon-reducing conditions. StemRD’sproduct is expressed from a human cell line in animal-free medium, and purifiedwith a proprietary process that is distinct from the published method.
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1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
现配现用

