| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | L-Kynurenine ELISA kit |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. |
| Format | 96-well plate |
| Samples | Plasma, serum, cell culture supernatant |
| Minimal sample volume | 20 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0/100 - 10000 ng/ml |
| Sensitivity | < 47.5 ng/ml |
| Specificity | No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid. |
| Assay time | Sample acylation 90 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage | Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | Sample acylation (90 min) |
| ELISA | L-Kynurenine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Product citations :
- Klar et al. A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression. Cancer Immunol Immunother. 2020 Jan
- Watanabe et al. Adding indoximod to hypofractionated radiotherapy with anti-PD-1 checkpoint blockade enhances early NK and CD8+ T cell-dependent tumor activity. Clin Cancer Res. 2019 Nov 6
- Hong et al. Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior. Asian J Psychiatr. 2019 Dec 19
- Allison et al. Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway. Am J Physiol Cell Physiol. 2019 Mar 1
- Qi et al. Lactobacillus maintains healthy gut mucosa by producing L-Ornithine Commun Biol. 2019 May 8
- Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.
- Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.
- Gautam et al. In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2018 Jan 2
- Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3
- Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
- Lee et al. Inhibition of acute lethal pulmonary inflammation by the IDO–AhR pathway. Proc Natl Acad Sci U S A. 2017 Jul 18
- Maliniemi et al. Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology. 2017; 6
- Escobar-Alvarez et al. The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function. Free Radic Biol Med. 2017 Nov
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没有采用RO反向渗透膜的水处理设备,基本上都可称为净水器。根据处理方法不同,有不同的叫法,如软水器、净水器等等。
功能:过虑掉自来水中所有物质,出来的水是纯净水,如果需要和矿泉水,可以单加一个麦饭石,这样出来的就是矿物质比较丰富的矿泉水了,当然,这样的矿泉水肯定比净水机出来的可以生饮的水好,净水机出来的水虽然有矿物质,但是只是自来水含有的,很多矿物质是自来水无法提供的。一句话:纯水机出来的水相当于外面卖的瓶装的纯净水,如果加上麦饭石,相当于卖的矿泉水;至于净水机,出来的水是有部分自来水中矿物质的可以生饮的净化水。
特点:有电机,需要电源,有储水罐,一般为五级过滤,第一级为滤芯,第二和第三级为活性炭,第四级为用于宇航技术的RO逆渗透膜(此为核心),第五级为精致活性炭,主要用于改善口感。可加第六级放置麦饭石。
净水机:
功能:能够过滤掉自来水氯、杂质等,最后出来的水能够达到生饮的标准,保留了自来水中的对人体有用的矿物质;
特点:没有电机,不需要电源,靠水压驱动过滤。没有储水罐,一般为五级过滤,第一级为滤芯,第二和第三级为活性炭,第四级为中空丝膜或陶瓷过滤,第五级为精致活性炭,主要用于改善口感。
直饮机
管道直饮机,是在饮水机的基础功能上增加进水自动控制器,使用时只需将管道直饮机与饮用水管道直接联接,实现自动进水,可直接饮用的饮水机。是现代住宅小区、写字楼供水的终端饮水设备。
我想咨询的是ELIX的处理柱(好象叫PRO-GUARD),多长时间换一次,我们这里在用水不是很大量的情况下半年就不行了,总是提示低压报警,现在刚换了三个月,又开始报警了,是否我们这里水质极差还是机器有问题?
该怎么解决呢,一个柱子近3000大洋,太贵了,还不如直接买纯净水划的来
请回答明确,谢谢
RCF = 1.119 x 10-5 x (rpm)2 x r
其中r 表示离心机转轴中心与离心管中心的距离,单位为cm。由于离心管的位置由转子(rotor)决定,因此r 必须由查阅相关转子的参数而得。

