
Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | L-Kynurenine ELISA kit |
Description | Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. |
Format | 96-well plate |
Samples | Plasma, serum, cell culture supernatant |
Minimal sample volume | 20 µL |
Cross specie-reactivity | React with all species |
Standard range | 0/100 - 10000 ng/ml |
Sensitivity | < 47.5 ng/ml |
Specificity | No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid. |
Assay time | Sample acylation 90 min and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
Sample collection & storage | Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
Sample preparation | Sample acylation (90 min) |
ELISA | L-Kynurenine antiserum overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
Product citations :
- Klar et al. A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression. Cancer Immunol Immunother. 2020 Jan
- Watanabe et al. Adding indoximod to hypofractionated radiotherapy with anti-PD-1 checkpoint blockade enhances early NK and CD8+ T cell-dependent tumor activity. Clin Cancer Res. 2019 Nov 6
- Hong et al. Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior. Asian J Psychiatr. 2019 Dec 19
- Allison et al. Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway. Am J Physiol Cell Physiol. 2019 Mar 1
- Qi et al. Lactobacillus maintains healthy gut mucosa by producing L-Ornithine Commun Biol. 2019 May 8
- Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.
- Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.
- Gautam et al. In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2018 Jan 2
- Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3
- Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
- Lee et al. Inhibition of acute lethal pulmonary inflammation by the IDO–AhR pathway. Proc Natl Acad Sci U S A. 2017 Jul 18
- Maliniemi et al. Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology. 2017; 6
- Escobar-Alvarez et al. The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function. Free Radic Biol Med. 2017 Nov
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纯水机的选取首先要看您是做什么用途?不同型号和配置的水机,在各个指标的纯化程度是有倾斜的,这也决定了各型号的水机采取什么核心组件,进而决定价格。例如,如果做的实验室细胞培养类型的,那么就要求有机物、无极离子都要净,价格相对较高;如果是无机分析实验,那么对水中的无机离子去除要求较高,有机物物质相对不那么严格,价格会略低。
同时还取决于设备的产量等因素。
进口的密理博、pall,价格都在5万以上,甚至十多万;国产的比较杂,近万到数万元不等。
国产中锐思捷技术比较好,价格在3-5万之间,其他的如和泰或者乐枫。
纯水机的价格影响因素除了进口品牌和国产品牌的差异外,主要取决于产量、用途以及其他特殊要求。
综合来看,除非有硬性规定或者需要采用进口品牌来提升实验室形象,否则建议选择国产品牌即可,因为实际上纯水机作为实验室常规设备,在工艺技术上知名度较高的国产品牌已足与进口相媲美,且价格仅为进口的1/3左右。
你需要先确定采购水机的主要实验用途,不同的实验用途,对水的要求是不一样的,从而也决定了所采购纯水机的配置,进而决定价格。
水中需纯化的物质主要有:无机离子、总有机碳(TOC)、微小粒子、微生物(内毒素、热原)、弱电解质。根据对以上物质的去除度,生产出来的纯水分为三个级别:初级纯水、中级水、超纯水。一般来水,仅产初级产水的纯水机价格在1.2万左右(产量10L);同时产初级纯水和超纯水的纯水机价格在(2-3万左右,产量10L)。
此外,还需要确定具体的实验用途,不同的实验用水,对于水中的物质去除度是有区分的,会有一定的倾斜度,比如细胞培养型实验,就对无机离子和TOC去除度要求严格;无机分析实验的话对无机离子去除要求高。
国产中,锐思捷、力康都还不错。
GB 19815-2005 离心机安全要求
GB 6065-1985 TZ立式振动离心机
GB 12258-1990 医用低速离心机
JB/T 3263-2000 卧式振动离心机
JB/T 4064-2005 上悬式离心机
JB/T 5519-1991高速冷冻离心机
JB/T 6118-1992 TCL沉降过滤离心机
JB/T 53190-1999 三足式离心机 产品质量分等
JB 447-1985卧式活塞推料离心机技术条件
JB/T 502-2004 螺旋卸料沉降离心机
JB/T 5284-1991 防爆型刮刀卸料离心机 序号 标 准 代 号 标 准 名 称 1 GB/T 4774-2004 分离机械 名词术语 2 GB 7779-2005 离心机型号编制方法 3 GB 10901-2005 离心机 性能测试方法 4 GB/19815-2005 离心机 安全要求 5 GB/T10894-2004 分离机械 噪音测试方法 6 GB/T10895-2004 离心机 分离机 机械振动测试方法 7 JB/T 447-2004 活塞推料离心机 8 JB/T 10411-2004 离心机、分离机奥氏体钢锻件超声检测及质量评级 9 JB/T 8051-2006 离心机转鼓强度计算规范 10 JB/T 8865-2001 活塞推料离心机用滤网 11 JB/T 9095-1999 离心机、分离机锻焊件无损探伤技术规范 12 JB/T 7217-2006 分离机械涂装通用技术条件 13 JB/T 6418-2006 分离机械 清洁度测定方法向左转|向右转
纯水机是属于科学仪器,用途在于实验室科研,工艺技术相较于净水器复杂很多,设备所用的组件和耗材也比净水器多,原则上可以说:净水器的超级升级版就是纯水机。
水中的杂质有微小颗粒、无机离子(钙离子、钠离子、镁离子等)、微生物(内毒素、热原)、弱电解质、有机物。
净水器是将水中的有害物质(污染物)净化成可直接饮用的水,但是水中仍有较多的无机离子和部分有机物,这对人体无害,甚至可被人体吸收利用;纯水机又分为初级纯水机和超纯水机,力求纯化后只剩下水,不含其它任何物质。
净水器的工艺是:预处理-反渗透;
纯水机的工艺是:预处理-反渗透-紫外消毒-DI离子交换-超滤(若需要);
净水器售价在几千元即可,最高不超过一万;纯水器可由近万元至十多万。
请回答明确,谢谢

