Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
Alternate Names
GFAP
Background Information
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Product Information
Format
Purified
Control
Mouse brain tissue, Astrocyte culture.
Presentation
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Storage and Shipping Information
Storage Conditions
Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt. DO NOT FREEZE.
Applications
Application
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.
Key Applications
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunocytochemistry: 5 μg/mL of a previous lot was used.
Immunohistochemistry: 5 µg/mL
Immunoblotting: Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified glial filament (Debus, E., 1983).
Clone
GA5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
Isotype
IgG1
Species Reactivity
Human
Rat
Mouse
Pig
Chicken
Bovine
Rabbit
Species Reactivity Note
Human, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. SIZE: 432 amino acids; 49880 Da SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1. SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments. DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.