Calbindin D28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. The biological roles of Calbindin D28k include, calcium regulation (including calcium transport and uptake, calcification of bone and teeth) and calcium related signalling in neurons and transiently in embryological development. This calbindin also has a role in protecting neurons from apoptotic cell death. Disregulation of this calbindin protein relates to several disease states. Decline in calbindin D28k in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D28k immunoreactivity is specifically absent from neuronal poplulations lost early in ALS, while substantial loss of neurons which do contain the protein occur in patients with Huntington's disease.
Product Information
Format
Purified
Presentation
Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% ProClin 300 as preservative.
Storage and Shipping Information
Storage Conditions
Maintain at 2-8oC. Refer to vial for expiration dating.
Applications
Application
Detect Calbindin D-28K using this Anti-Calbindin D-28K Antibody, prediluted validated for use in IH(P).
Key Applications
Immunohistochemistry (Paraffin)
Application Notes
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.
Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.
Incubation: 30 minutes with IHC Select® Detection Kits.
Calbindin has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1013-6.
Biological Information
Immunogen
Purified recombinant calbindin.
Host
Rabbit
Specificity
Calbindin. Shows no cross-reactivity to calretinin by Western blot. The antibody produces specific staining of cerebellum Purkinje cells, molecular layer dendrites and axonal fibers, stains cell bodies and fibers in neuronal subpopulations.
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted.
FUNCTION: SwissProt: P05937 # Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. SIZE: 261 amino acids; 30025 Da SUBUNIT: Interacts with RANBP9. DOMAIN: SwissProt: P05937 This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. SIMILARITY: Belongs to the calbindin family. & Contains 5 EF-hand domains.