The human IL-1α ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IL-1α in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
The assay is specific for human IL-1α (pro-form and active form) and shows no cross-reactivity with recombinant human IL-1β or IL-18.
Human IL-1α ELISABASIC kits are also available with Streptavidin-HRP. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-1αInterleukin-1α (IL-1α, also known as hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α and Interleukin-1 alpha) is a proinflammatory cytokine and inducer of acute phase responses. IL-1α is produced primarily by monocytes, macrophages, and neutrophil after induction by bacterial product such as LPS.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order to order@mabtech.com.
Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.Application: |
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Reactivity: |
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Standard range: | 2-200 pg/ml |
Documentation: | Datasheet/ProtocolSDS |
Contents: |
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Size: | for 6 plates |
Other formats: | Click to view all Human IL-1α products |
Very easy to use ELISA kit. |
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血清内含有抗体,封闭的时候,选用同物种的血清,比如,使用小鼠单抗的话,就选用小鼠血清来进行封闭。这样,如果细胞表面有可以非特异性结合小鼠抗体的部位都会事先被结合,就不会再结合荧光标记的特异性抗体了。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。